The Composition of the Microbiota in the Full-Term Fetal Gut and Amniotic Fluid : A Bovine Cesarean Section Study

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Husso , A , Lietaer , L , Pessa-Morikawa , T , Grönthal , T , Govaere , J , Van Soom , A , Iivanainen , A , Opsomer , G & Niku , M 2021 , ' The Composition of the Microbiota in the Full-Term Fetal Gut and Amniotic Fluid : A Bovine Cesarean Section Study ' , Frontiers in Microbiology , vol. 12 , 626421 . https://doi.org/10.3389/fmicb.2021.626421

Title: The Composition of the Microbiota in the Full-Term Fetal Gut and Amniotic Fluid : A Bovine Cesarean Section Study
Author: Husso, Aleksi; Lietaer, Leen; Pessa-Morikawa, Tiina; Grönthal, Thomas; Govaere, Jan; Van Soom, Ann; Iivanainen, Antti; Opsomer, Geert; Niku, Mikael
Contributor organization: Helsinki One Health (HOH)
Departments of Faculty of Veterinary Medicine
Veterinary Biosciences
Developmental interactions
Antti Iivanainen / Principal Investigator
Veterinary Anatomy and Developmental Biology
Equine and Small Animal Medicine
Fetal microbiota
Teachers' Academy
Date: 2021-04-30
Language: eng
Number of pages: 11
Belongs to series: Frontiers in Microbiology
ISSN: 1664-302X
DOI: https://doi.org/10.3389/fmicb.2021.626421
URI: http://hdl.handle.net/10138/330331
Abstract: The development of a healthy intestinal immune system requires early microbial exposure. However, it remains unclear whether microbial exposure already begins at the prenatal stage. Analysis of such low microbial biomass environments are challenging due to contamination issues. The aims of the current study were to assess the bacterial load and characterize the bacterial composition of the amniotic fluid and meconium of full-term calves, leading to a better knowledge of prenatal bacterial seeding of the fetal intestine. Amniotic fluid and rectal meconium samples were collected during and immediately after elective cesarean section, performed in 25 Belgian Blue cow-calf couples. The samples were analyzed by qPCR, bacterial culture using GAM agar and 16S rRNA gene amplicon sequencing. To minimize the effects of contaminants, we included multiple technical controls and stringently filtered the 16S rRNA gene sequencing data to exclude putative contaminant sequences. The meconium samples contained a significantly higher amount of bacterial DNA than the negative controls and 5 of 24 samples contained culturable bacteria. In the amniotic fluid, the amount of bacterial DNA was not significantly different from the negative controls and all samples were culture negative. Bacterial sequences were identified in both sample types and were primarily of phyla Proteobacteria, Firmicutes, Bacteroidetes, and Actinobacteria, with some individual variation. We conclude that most calves encounter in utero maternal-fetal transmission of bacterial DNA, but the amount of bacterial DNA is low and viable bacteria are rare.
Subject: bovine
gut
amniotic fluid
meconium
fetal
cesarean section
microbiome
neonatal
PERINATAL INTESTINAL MICROBIOTA
BACTERIAL LOAD
STERILE WOMB
IN-UTERO
COLONIZATION
MECONIUM
CONTAMINATION
COMMUNITIES
EXTRACTION
PLACENTA
413 Veterinary science
Peer reviewed: Yes
Rights: cc_by
Usage restriction: openAccess
Self-archived version: publishedVersion


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