Bacterial diversity and predicted enzymatic function in a multipurpose surface water system – from wastewater effluent discharges to drinking water production

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Tiwari , A , Hokajärvi , A M , Domingo , J S , Elk , M , Jayaprakash , B , Ryu , H , Siponen , S , Vepsäläinen , A , Kauppinen , A , Puurunen , O , Artimo , A , Perkola , N , Huttula , T , Miettinen , I T & Pitkänen , T 2021 , ' Bacterial diversity and predicted enzymatic function in a multipurpose surface water system – from wastewater effluent discharges to drinking water production ' , Environmental microbiome , vol. 16 , no. 1 , 11 .

Title: Bacterial diversity and predicted enzymatic function in a multipurpose surface water system – from wastewater effluent discharges to drinking water production
Author: Tiwari, Ananda; Hokajärvi, Anna Maria; Domingo, Jorge Santo; Elk, Michael; Jayaprakash, Balamuralikrishna; Ryu, Hodon; Siponen, Sallamaari; Vepsäläinen, Asko; Kauppinen, Ari; Puurunen, Osmo; Artimo, Aki; Perkola, Noora; Huttula, Timo; Miettinen, Ilkka T.; Pitkänen, Tarja
Contributor organization: Food Hygiene and Environmental Health
Zoonotic Antimicrobial Resistance
Faculty of Veterinary Medicine
University of Helsinki
Helsinki One Health (HOH)
Waterborne pathogens
Doctoral Programme in Food Chain and Health
Date: 2021-12
Language: eng
Number of pages: 17
Belongs to series: Environmental microbiome
ISSN: 2524-6372
Abstract: Background Rivers and lakes are used for multiple purposes such as for drinking water (DW) production, recreation, and as recipients of wastewater from various sources. The deterioration of surface water quality with wastewater is well-known, but less is known about the bacterial community dynamics in the affected surface waters. Understanding the bacterial community characteristics -from the source of contamination, through the watershed to the DW production process-may help safeguard human health and the environment. Results The spatial and seasonal dynamics of bacterial communities, their predicted functions, and potential health-related bacterial (PHRB) reads within the Kokemaenjoki River watershed in southwest Finland were analyzed with the 16S rRNA-gene amplicon sequencing method. Water samples were collected from various sampling points of the watershed, from its major pollution sources (sewage influent and effluent, industrial effluent, mine runoff) and different stages of the DW treatment process (pre-treatment, groundwater observation well, DW production well) by using the river water as raw water with an artificial groundwater recharge (AGR). The beta-diversity analysis revealed that bacterial communities were highly varied among sample groups (R = 0.92, p <0.001, ANOSIM). The species richness and evenness indices were highest in surface water (Chao1; 920 +/- 10) among sample groups and gradually decreased during the DW treatment process (DW production well; Chao1: 320 +/- 20). Although the phylum Proteobacteria was omnipresent, its relative abundance was higher in sewage and industrial effluents (66-80%) than in surface water (55%). Phyla Firmicutes and Fusobacteria were only detected in sewage samples. Actinobacteria was more abundant in the surface water (>= 13%) than in other groups (= 13%) than in others (
Description: Funding Information: The authors would like to express special acknowledgment to the CONPAT research team at the Finnish Institute for Health and Welfare, Finnish Environment Institute, and VATT Institute for Economic Research. Special thanks go to Tiina Heiskanen and Laura Wessels for extracting the nucleic acids. The Water Protection Association of the River Kokem?enjoki (KVVY) is acknowledged for surface water and wastewater sampling. Funding Information: Academy of Finland (grant number 263451) and Kaute Foundation (grant number 20190366) are acknowledged for providing funds for the project establishment and manuscript writing, respectively. Funding Information: The authors declare that they have no competing interests. This work was in part supported by the U.S. Environmental Protection Agency (EPA), and any opinions expressed do not reflect the views of the agency; therefore, no official endorsement should be inferred. Any mention of trade names or commercial products does not constitute endorsement or recommendation for use. Publisher Copyright: © 2021, The Author(s).
Subject: Bacterial communities
Sewage effluent
Surface water
Seasonal effects
Predicted biological function
16S rRNA amplicon sequencing
3142 Public health care science, environmental and occupational health
11832 Microbiology and virology
Peer reviewed: Yes
Rights: cc_by
Usage restriction: openAccess
Self-archived version: publishedVersion

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