UriSed 3 PRO automated microscope in screening bacteriuria at region-wide laboratory organization

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http://hdl.handle.net/10138/330616

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Kouri , T , Holma , T , Kirjavainen , V , Lempiäinen , A , Alagrund , K , Tohmola , N , Pihlajamaa , T , Kouri , V-P , Lehtonen , M , Friman , S & Patari-Sampo , A 2021 , ' UriSed 3 PRO automated microscope in screening bacteriuria at region-wide laboratory organization ' , Clinica Chimica Acta , vol. 516 , pp. 149-156 . https://doi.org/10.1016/j.cca.2021.01.022

Title: UriSed 3 PRO automated microscope in screening bacteriuria at region-wide laboratory organization
Author: Kouri, Timo; Holma, Tanja; Kirjavainen, Vesa; Lempiäinen, Anna; Alagrund, Katariina; Tohmola, Niina; Pihlajamaa, Tero; Kouri, Vesa-Petteri; Lehtonen, Maaret; Friman, Sirpa; Patari-Sampo, Anu
Contributor organization: Medicum
HUSLAB
Department of Clinical Chemistry and Hematology
Department of Diagnostics and Therapeutics
Clinicum
HUS Internal Medicine and Rehabilitation
Date: 2021-05
Language: eng
Number of pages: 8
Belongs to series: Clinica Chimica Acta
ISSN: 0009-8981
DOI: https://doi.org/10.1016/j.cca.2021.01.022
URI: http://hdl.handle.net/10138/330616
Abstract: Background and aims: We assessed the possibility to rule out negative urine cultures by counting with UriSed 3 PRO (77 Elektmnika, Hungary) at Helsinki and Uusimaa Hospital District. Materials and methods: Bacteria counting of the UriSed 3 PRO automated microscope was verified with reference phase contrast microscopy against growth in culture. After acceptance into routine, results of bacteria and leukocyte counting from 56 426 specimens with eight UriSed 3 PRO instruments were compared against results from parallel samples cultured on chromogenic agar. Laboratory data including preanalytical details were accessed through the regional database of the Helsinki and Uusimaa Hospital District. Results: A combined sensitivity of 87-92% and a negative predictive value of 90-96% with a specificity of 54-50% was reached, depending on criteria. Preanalytical data (incubation time in bladder) combined with the way of urine collection would improve these figures if reliable. Conclusions: Complex patient populations, regional logistics and data interfases, and economics related to increased costs of additional particle counts against costs of screening cultures of all samples, did not support adaptation of a screening process of urine cultures. This conclusion was made locally, and may not be valid elsewhere.
Subject: Automation
Bacteria screening
Urinary tract infection
Urine culture
Urine particles
UriSed 3 PRO
3111 Biomedicine
Peer reviewed: Yes
Rights: cc_by
Usage restriction: openAccess
Self-archived version: publishedVersion


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