HLA RNA Sequencing With Unique Molecular Identifiers Reveals High Allele-Specific Variability in mRNA Expression

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Johansson , T , Yohannes , D A , Koskela , S , Partanen , J & Saavalainen , P 2021 , ' HLA RNA Sequencing With Unique Molecular Identifiers Reveals High Allele-Specific Variability in mRNA Expression ' , Frontiers in Immunology , vol. 12 , 629059 . https://doi.org/10.3389/fimmu.2021.629059

Title: HLA RNA Sequencing With Unique Molecular Identifiers Reveals High Allele-Specific Variability in mRNA Expression
Author: Johansson, Tiira; Yohannes, Dawit A.; Koskela, Satu; Partanen, Jukka; Saavalainen, Paivi
Contributor organization: HUMI - Human Microbiome Research
Biosciences
Immunomics
University Management
Date: 2021-02-25
Language: eng
Number of pages: 14
Belongs to series: Frontiers in Immunology
ISSN: 1664-3224
DOI: https://doi.org/10.3389/fimmu.2021.629059
URI: http://hdl.handle.net/10138/330669
Abstract: The HLA gene complex is the most important single genetic factor in susceptibility to most diseases with autoimmune or autoinflammatory origin and in transplantation matching. Most studies have focused on the vast allelic variation in these genes; only a few studies have explored differences in the expression levels of HLA alleles. In this study, we quantified mRNA expression levels of HLA class I and II genes from peripheral blood samples of 50 healthy individuals. The gene- and allele-specific mRNA expression was assessed using unique molecular identifiers, which enabled PCR bias removal and calculation of the number of original mRNA transcripts. We identified differences in mRNA expression between different HLA genes and alleles. Our results suggest that HLA alleles are differentially expressed and these differences in expression levels are quantifiable using RNA sequencing technology. Our method provides novel insights into HLA research, and it can be applied to quantify expression differences of HLA alleles in various tissues and to evaluate the role of this type of variation in transplantation matching and susceptibility to autoimmune diseases.
Subject: HLA
allele-specific expression
RNA sequencing
unique molecular identifiers
gene expression
3121 General medicine, internal medicine and other clinical medicine
Peer reviewed: Yes
Rights: cc_by
Usage restriction: openAccess
Self-archived version: publishedVersion


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