Phage Biocontrol of Pseudomonas aeruginosa in Water

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Kauppinen , A , Siponen , S , Pitkänen , T , Holmfeldt , K , Pursiainen , A , Torvinen , E & Miettinen , I T 2021 , ' Phage Biocontrol of Pseudomonas aeruginosa in Water ' , Viruses (Basel) , vol. 13 , no. 5 , 928 . https://doi.org/10.3390/v13050928

Title: Phage Biocontrol of Pseudomonas aeruginosa in Water
Author: Kauppinen, Ari; Siponen, Sallamaari; Pitkänen, Tarja; Holmfeldt, Karin; Pursiainen, Anna; Torvinen, Eila; Miettinen, Ilkka T.
Contributor organization: Helsinki One Health (HOH)
Waterborne pathogens
Food Hygiene and Environmental Health
Date: 2021-05
Language: eng
Number of pages: 16
Belongs to series: Viruses (Basel)
ISSN: 1999-4915
DOI: https://doi.org/10.3390/v13050928
URI: http://hdl.handle.net/10138/330809
Abstract: Bacteriophage control of harmful or pathogenic bacteria has aroused growing interest, largely due to the rise of antibiotic resistance. The objective of this study was to test phages as potential agents for the biocontrol of an opportunistic pathogen Pseudomonas aeruginosa in water. Two P. aeruginosa bacteriophages (vB_PaeM_V523 and vB_PaeM_V524) were isolated from wastewater and characterized physically and functionally. Genomic and morphological characterization showed that both were myoviruses within the Pbunavirus genus. Both had a similar latent period (50-55 min) and burst size (124-134 PFU/infected cell), whereas there was variation in the host range. In addition to these environmental phages, a commercial Pseudomonas phage, JG003 (DSM 19870), was also used in the biocontrol experiments. The biocontrol potential of the three phages in water was tested separately and together as a cocktail against two P. aeruginosa strains; PAO1 and the environmental strain 17V1507. With PAO1, all phages initially reduced the numbers of the bacterial host, with phage V523 being the most efficient (>2.4 log(10) reduction). For the environmental P. aeruginosa strain (17V1507), only the phage JG003 caused a reduction (1.2 log(10)) compared to the control. The cocktail of three phages showed a slightly higher decrease in the level of the hosts compared to the use of individual phages. Although no synergistic effect was observed in the host reduction with the use of the phage cocktail, the cocktail-treated hosts did not appear to acquire resistance as rapidly as hosts treated with a single phage. The results of this study provide a significant step in the development of bacteriophage preparations for the control of pathogens and harmful microbes in water environments.
Subject: Pseudomonas aeruginosa
bacteriophages
biocontrol
phage cocktail
water treatment
BACTERIOPHAGES
OUTBREAK
RESISTANCE
CONTAMINATION
STRATEGIES
SEQUENCE
GENE
FOOD
PAO1
11832 Microbiology and virology
3142 Public health care science, environmental and occupational health
Peer reviewed: Yes
Rights: cc_by
Usage restriction: openAccess
Self-archived version: publishedVersion


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