Genomic diversity of Listeria monocytogenes isolates from seafood, horticulture and factory environments in New Zealand

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Mohan , V , Cruz , C D , van Vliet , A H M , Pitman , A R , Visnovsky , S B , Rivas , L , Gilpin , B & Fletcher , G C 2021 , ' Genomic diversity of Listeria monocytogenes isolates from seafood, horticulture and factory environments in New Zealand ' , International Journal of Food Microbiology , vol. 347 , 109166 . https://doi.org/10.1016/j.ijfoodmicro.2021.109166

Title: Genomic diversity of Listeria monocytogenes isolates from seafood, horticulture and factory environments in New Zealand
Author: Mohan, Vathsala; Cruz, Cristina D.; van Vliet, Arnoud H. M.; Pitman, Andrew R.; Visnovsky, Sandra B.; Rivas, Lucia; Gilpin, Brent; Fletcher, Graham C.
Contributor organization: Division of Pharmaceutical Biosciences
Drug Research Program
Date: 2021-06-02
Language: eng
Number of pages: 13
Belongs to series: International Journal of Food Microbiology
ISSN: 0168-1605
DOI: https://doi.org/10.1016/j.ijfoodmicro.2021.109166
URI: http://hdl.handle.net/10138/330825
Abstract: Listeria monocytogenes is a foodborne human pathogen that causes systemic infection, fetal-placental infection in pregnant women causing abortion and stillbirth and meningoencephalitis in elderly and immunocompromised individuals. This study aimed to analyse L. monocytogenes from different sources from New Zealand (NZ) and to compare them with international strains. We used pulsed-field gel electrophoresis (PFGE), multilocus sequence typing (MLST) and whole-genome single nucleotide polymorphisms (SNP) to study the population structure of the NZ L. monocytogenes isolates and their relationship with the international strains. The NZ isolates formed unique clusters in PFGE, MLST and whole-genome SNP comparisons compared to the international isolates for which data were available. PFGE identified 31 AscI and 29 ApaI PFGE patterns with indistinguishable pulsotypes being present in seafood, horticultural products and environmental samples. Apart from the Asc0002:Apa0002 pulsotype which was distributed across different sources, other pulsotypes were site or factory associated. Wholegenome analysis of 200 randomly selected L. monocytogenes isolates revealed that lineage II dominated the NZ L. monocytogenes populations. MLST comparison of international and NZ isolates with lineage II accounted for 89% (177 of 200) of the total L. monocytogenes population, while the international representation was 45.3% (1674 of 3473). Rarefaction analysis showed that sequence type richness was greater in NZ isolates compared to international trend, however, it should be noted that NZ isolates predominantly came from seafood, horticulture and their respective processing environments or factories, unlike international isolates where there was a good mixture of clinical, food and environmental isolates.
Subject: Genomic diversity
Listeria monocytogenes
Seafood
Horticulture and factory environments
PFGE
MLST
WGS
New Zealand
FIELD GEL-ELECTROPHORESIS
OUTBREAK
STRAINS
CLONES
MEAT
SURVEILLANCE
INFECTION
BIOFILM
ENGLAND
HUMANS
11832 Microbiology and virology
416 Food Science
3142 Public health care science, environmental and occupational health
317 Pharmacy
Peer reviewed: Yes
Rights: cc_by_nc_nd
Usage restriction: openAccess
Self-archived version: publishedVersion


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