Mini-Intein Structures from Extremophiles Suggest a Strategy for Finding Novel Robust Inteins

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http://hdl.handle.net/10138/330857

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Hiltunen, M.K.; Beyer, H.M.; Iwaï, H. Mini-Intein Structures from Extremophiles Suggest a Strategy for Finding Novel Robust Inteins. Microorganisms 2021, 9, 1226.

Title: Mini-Intein Structures from Extremophiles Suggest a Strategy for Finding Novel Robust Inteins
Author: Hiltunen, Mimmu K.; Beyer, Hannes M.; Iwaï, Hideo
Publisher: Multidisciplinary Digital Publishing Institute
Date: 2021-06-05
URI: http://hdl.handle.net/10138/330857
Abstract: Inteins are prevalent among extremophiles. Mini-inteins with robust splicing properties are of particular interest for biotechnological applications due to their small size. However, biochemical and structural characterization has still been limited to a small number of inteins, and only a few serve as widely used tools in protein engineering. We determined the crystal structure of a naturally occurring Pol-II mini-intein from <i>Pyrococcus horikoshii</i> and compared all three mini-inteins found in the genome of <i>P. horikoshii</i>. Despite their similar sizes, the comparison revealed distinct differences in the insertions and deletions, implying specific evolutionary pathways from distinct ancestral origins. Our studies suggest that sporadically distributed mini-inteins might be more promising for further protein engineering applications than highly conserved mini-inteins. Structural investigations of additional inteins could guide the shortest path to finding novel robust mini-inteins suitable for various protein engineering purposes.


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