An aMMP-8 Point-of-Care and Questionnaire Based Real-Time Diagnostic Toolkit for Medical Practitioners

Show simple item record Räisänen, Ismo T. Lähteenmäki, Hanna Gupta, Shipra Grigoriadis, Andreas Sahni, Vaibhav Suojanen, Juho Seppänen, Hanna Tervahartiala, Taina Sakellari, Dimitra Sorsa, Timo 2021-06-15T07:54:01Z 2021-06-15T07:54:01Z 2021-04
dc.identifier.citation Räisänen , I T , Lähteenmäki , H , Gupta , S , Grigoriadis , A , Sahni , V , Suojanen , J , Seppänen , H , Tervahartiala , T , Sakellari , D & Sorsa , T 2021 , ' An aMMP-8 Point-of-Care and Questionnaire Based Real-Time Diagnostic Toolkit for Medical Practitioners ' , Diagnostics , vol. 11 , no. 4 , 711 .
dc.identifier.other PURE: 165169667
dc.identifier.other PURE UUID: fc3dcfe6-e522-434c-adf3-615aec9a2e3e
dc.identifier.other WOS: 000642988500001
dc.identifier.other ORCID: /0000-0002-2361-5134/work/95558552
dc.identifier.other ORCID: /0000-0001-5821-5299/work/95560279
dc.description.abstract The aim of this cross-sectional study is to propose an efficient strategy based on biomarkers adjunct with an interview/questionnaire covering risk factors for periodontitis for the identification of undiagnosed periodontitis by medical professionals. Active matrix metalloproteinase (aMMP)-8 levels in mouthrinse were analyzed by a point-of-care (PoC)/chairside lateral-flow immunotest, and salivary total MMP-8, total MMP-9 and calprotectin levels were analyzed by enzyme-linked immunosorbent assays (ELISAs) and active MMP-9 by gelatin zymography for 149 Greek patients. Patients underwent a full-mouth oral health examination for diagnosis according to the 2018 classification system of periodontal diseases. In addition, patient characteristics (risk factors: age, gender, education level, smoking and body mass index) were recorded. Receiver operating curve (ROC) analysis indicated better diagnostic precision to identify undiagnosed periodontitis for oral fluid biomarkers in adjunct with an interview/questionnaire compared with a plain questionnaire (i.e., risk factors): aMMP-8 AUC (95% confidence interval) = 0.834 (0.761-0.906), total MMP-8 = 0.800 (0.722-0.878), active MMP-9 = 0.787 (0.704-0.870), total MMP-9 = 0.773 (0.687-0.858) and calprotectin = 0.773 (0.687-0.858) vs. questionnaire = 0.764 (0.676-0.851). The findings of this study suggest that oral fluid biomarker analysis, such as a rapid aMMP-8 PoC immunotest, could be used as an adjunct to an interview/questionnaire to improve the precision of timely identification of asymptomatic, undiagnosed periodontitis patients by medical professionals. This strategy appears to be viable for referring patients to a dentist for diagnosis and treatment need assessment. en
dc.format.extent 8
dc.language.iso eng
dc.relation.ispartof Diagnostics
dc.rights cc_by
dc.rights.uri info:eu-repo/semantics/openAccess
dc.subject periodontitis
dc.subject diagnostics
dc.subject point-of-care systems
dc.subject biomarkers
dc.subject matrix metalloproteinase 8
dc.subject preventive medicine
dc.subject oral health
dc.subject 316 Nursing
dc.title An aMMP-8 Point-of-Care and Questionnaire Based Real-Time Diagnostic Toolkit for Medical Practitioners en
dc.type Article
dc.contributor.organization Doctoral Programme in Oral Sciences
dc.contributor.organization Department of Oral and Maxillofacial Diseases
dc.contributor.organization University of Helsinki
dc.contributor.organization Helsinki University Hospital Area
dc.contributor.organization Plastiikkakirurgian yksikkö
dc.contributor.organization Clinicum
dc.contributor.organization HUS Head and Neck Center
dc.contributor.organization Päijät-Häme Welfare Consortium
dc.contributor.organization CAN-PRO - Translational Cancer Medicine Program
dc.contributor.organization HUS Abdominal Center
dc.contributor.organization II kirurgian klinikka
dc.contributor.organization Department of Surgery
dc.description.reviewstatus Peer reviewed
dc.relation.issn 2075-4418
dc.rights.accesslevel openAccess
dc.type.version publishedVersion

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