Adverse prognostic impact of regulatory T-cells in testicular diffuse large B-cell lymphoma

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dc.contributor.author Pollari, Marjukka
dc.contributor.author Pellinen, Teijo
dc.contributor.author Karjalainen-Lindsberg, Marja-Liisa
dc.contributor.author Kellokumpu-Lehtinen, Pirkko-Liisa
dc.contributor.author Leivonen, Suvi-Katri
dc.contributor.author Leppä, Sirpa
dc.date.accessioned 2021-07-05T22:30:52Z
dc.date.available 2021-12-18T03:45:10Z
dc.date.issued 2020-12
dc.identifier.citation Pollari , M , Pellinen , T , Karjalainen-Lindsberg , M-L , Kellokumpu-Lehtinen , P-L , Leivonen , S-K & Leppä , S 2020 , ' Adverse prognostic impact of regulatory T-cells in testicular diffuse large B-cell lymphoma ' , European Journal of Haematology , vol. 105 , no. 6 , pp. 712-721 . https://doi.org/10.1111/ejh.13484
dc.identifier.other PURE: 149235826
dc.identifier.other PURE UUID: e9518583-2594-4033-87e0-56ecfc7caff6
dc.identifier.other WOS: 000571594200001
dc.identifier.other ORCID: /0000-0002-8265-511X/work/85519339
dc.identifier.other ORCID: /0000-0003-3224-0757/work/85519639
dc.identifier.uri http://hdl.handle.net/10138/332255
dc.description.abstract Objectives Testicular diffuse large B-cell lymphoma (T-DLBCL) is a rare and aggressive extranodal lymphoma. We have previously shown that high content of tumor-infiltrating lymphocytes (TILs) and PD-1 expressing TILs associate with better survival in T-DLBCL. In this study, we have further characterized distinct TIL subtypes and their proportions in association with patient demographics and survival. Methods We used multiplex immunohistochemistry to characterize TIL phenotypes, including cytotoxic T-cells (CTLs; CD8(+), OX40(+), Granzyme B+, Ki-67(+), LAG-3(+), TIM-3(+), PD-1(+)), CD4(+)T-cells (CD3(+), CD4(+), TIM-3(+), LAG-3(+)), regulatory T-cells (Tregs; CD3(+), CD4(+), FoxP3(+)), and T helper 1 cells (Th1; CD3(+), CD4(+), T-bet(+)) in 79 T-DLBCLs, and correlated the findings with patient demographics and outcome. Results We observed a substantial variation in TIL phenotypes between the patients. The most prominent CD8(+)TILs were Ki-67(+)and TIM-3(+)CTLs, whereas the most prominent CD4(+)TILs were FoxP3(+)Tregs. Despite the overall favorable prognostic impact of high TIL content, we found a subpopulation of T-bet(+)FoxP3(+)Tregs that had a significant adverse impact on survival. Lower content of CTLs with activated or exhausted phenotypes correlated with aggressive clinical features. Conclusions Our results demonstrate significant variation in TIL phenotypes and emphasize the adverse prognostic impact of Tregs in T-DLBCL. en
dc.format.extent 10
dc.language.iso eng
dc.relation.ispartof European Journal of Haematology
dc.rights.uri info:eu-repo/semantics/openAccess
dc.subject lymphoma
dc.subject regulatory T-cell
dc.subject testicular diffuse large B-cell lymphoma
dc.subject tumor-infiltrating lymphocyte
dc.subject CENTRAL-NERVOUS-SYSTEM
dc.subject CLASS-II EXPRESSION
dc.subject TUMOR MICROENVIRONMENT
dc.subject TRANSCRIPTION FACTOR
dc.subject FEATURES
dc.subject HODGKIN
dc.subject PD-1
dc.subject BET
dc.subject TOLERANCE
dc.subject BLOCKADE
dc.subject 3122 Cancers
dc.title Adverse prognostic impact of regulatory T-cells in testicular diffuse large B-cell lymphoma en
dc.type Article
dc.contributor.organization Research Programs Unit
dc.contributor.organization Faculty of Medicine
dc.contributor.organization University of Helsinki
dc.contributor.organization Precision Systems Medicine
dc.contributor.organization Institute for Molecular Medicine Finland
dc.contributor.organization HUS Comprehensive Cancer Center
dc.contributor.organization Medicum
dc.contributor.organization Department of Pathology
dc.contributor.organization Helsinki University Hospital Area
dc.contributor.organization Genome-Scale Biology (GSB) Research Program
dc.contributor.organization Department of Oncology
dc.contributor.organization Clinicum
dc.contributor.organization Digital Precision Cancer Medicine (iCAN)
dc.description.reviewstatus Peer reviewed
dc.relation.doi https://doi.org/10.1111/ejh.13484
dc.relation.issn 0902-4441
dc.rights.accesslevel openAccess
dc.type.version acceptedVersion

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