Electrokinetic characterization of extracellular vesicles with capillary electrophoresis : A new tool for their identification and quantification

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http://hdl.handle.net/10138/332354

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Morani , M , Duc Mai , T , Krupova , Z , Defrenaix , P , Multia , E , Riekkola , M-L & Taverna , M 2020 , ' Electrokinetic characterization of extracellular vesicles with capillary electrophoresis : A new tool for their identification and quantification ' , Analytica Chimica Acta , vol. 1128 , pp. 42-51 . https://doi.org/10.1016/j.aca.2020.06.073

Title: Electrokinetic characterization of extracellular vesicles with capillary electrophoresis : A new tool for their identification and quantification
Author: Morani, Marco; Duc Mai, Thanh; Krupova, Zuzana; Defrenaix, Pierre; Multia, Evgen; Riekkola, Marja-Liisa; Taverna, Myriam
Contributor: University of Helsinki, Department of Chemistry
University of Helsinki, Department of Chemistry
Date: 2020-09-01
Language: eng
Number of pages: 10
Belongs to series: Analytica Chimica Acta
ISSN: 0003-2670
URI: http://hdl.handle.net/10138/332354
Abstract: This work reports on the development of the first capillary electrophoresis methodology for the elucidation of extracellular vesicles' (EVs) electrokinetic distributions. The approach is based on capillary electrophoresis coupled with laser-induced fluorescent (LIF) detection for the identification and quantification of EVs after their isolation. Sensitive detection of these nanometric entities was possible thanks to an 'inorganic-species-free' background electrolyte. This electrolyte was made up of weakly charged molecules at very high concentrations to stabilize EVs, and an intra-membrane labelling approach was used to prevent EV morphology modification. The limit of detection for EVs achieved using the developed CE-LIF method reached 8 x 10(9) EV/mL, whereas the calibration curve was acquired from 1.22 x 10(10) to 1.20 x 10(11) EV/mL. The CE-LIF approach was applied to provide the electrokinetic distributions of various EVs of animal and human origins, and visualize different EV subpopulations from our recently developed high-yield EV isolation method. (C) 2020 Elsevier B.V. All rights reserved.
Subject: Capillary electrophoresis
LIF detection
Membrane labeling
Extracellular vesicles
FLOW-CYTOMETRY
SEPARATION
PARTICLES
NANOPARTICLES
SIZE
EXOSOMES
BEHAVIOR
MARKERS
116 Chemical sciences
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