Rapid quantification of microalgae growth with hyperspectral camera and vegetation indices

Show full item record



Permalink

http://hdl.handle.net/10138/332402

Citation

Salmi, P.; Eskelinen, M.A.; Leppänen, M.T.; Pölönen, I. Rapid Quantification of Microalgae Growth with Hyperspectral Camera and Vegetation Indices. Plants 2021, 10, 341. https://doi.org/10.3390/plants10020341

Files in this item

Total number of downloads: Loading...

Files Size Format View Description
Salmi et al. 20 ... and vegetation indices.pdf 3.933Mb PDF View/Open
Salmi et al. 2021 Supplementary material.pdf 713.8Kb PDF View/Open Supplementary material
Title: Rapid quantification of microalgae growth with hyperspectral camera and vegetation indices
Author: Salmi, Pauliina; Eskelinen, Matti A.; Leppänen, Matti T.; Pölönen, Ilkka
Publisher: MDPI AG
Date: 2021
Language: en
Belongs to series: Plants 2021, 10(2), 341
ISSN: 2223-7747
URI: http://hdl.handle.net/10138/332402
Abstract: Spectral cameras are traditionally used in remote sensing of microalgae, but increasingly also in laboratory-scale applications, to study and monitor algae biomass in cultures. Practical and cost-efficient protocols for collecting and analyzing hyperspectral data are currently needed. The purpose of this study was to test a commercial, easy-to-use hyperspectral camera to monitor the growth of different algae strains in liquid samples. Indices calculated from wavebands from transmission imaging were compared against algae abundance and wet biomass obtained from an electronic cell counter, chlorophyll a concentration, and chlorophyll fluorescence. A ratio of selected wavebands containing near-infrared and red turned out to be a powerful index because it was simple to calculate and interpret, yet it yielded strong correlations to abundances strain-specifically (0.85 < r < 0.96, p < 0.001). When all the indices formulated as A/B, A/(A + B) or (A − B)/(A + B), where A and B were wavebands of the spectral camera, were scrutinized, good correlations were found amongst them for biomass of each strain (0.66 < r < 0.98, p < 0.001). Comparison of near-infrared/red index to chlorophyll a concentration demonstrated that small-celled strains had higher chlorophyll absorbance compared to strains with larger cells. The comparison of spectral imaging to chlorophyll fluorescence was done for one strain of green algae and yielded strong correlations (near-infrared/red, r = 0.97, p < 0.001). Consequently, we described a simple imaging setup and information extraction based on vegetation indices that could be used to monitor algae cultures.
Subject: mobile spectral camera
vegetation indices
monitoring
transmission imaging
algae
microalgae
growth
vegetation
chlorophyll
biomass
green algae
state of the environment
remote sensing
strain
Subject (ysa): mikrolevät
levät
monitorointi
kasvillisuus
kaukokartoitus
spektrikuvaus
klorofylli
biomassa (ekologia)
fluoresenssi
kaukokartoitus
viherlevät
ympäristön tila
rasitus


This item appears in the following Collection(s)

Show full item record