Electrochemical Detection of Morphine in Untreated Human Capillary Whole Blood

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Verrinder , E , Wester , N , Leppänen , E , Lilius , T , Kalso , E , Mikladal , B , Varjos , I , Koskinen , J & Laurila , T 2021 , ' Electrochemical Detection of Morphine in Untreated Human Capillary Whole Blood ' , ACS Omega , vol. 6 , no. 17 , pp. 11563-11569 . https://doi.org/10.1021/acsomega.1c00773

Title: Electrochemical Detection of Morphine in Untreated Human Capillary Whole Blood
Author: Verrinder, Elsi; Wester, Niklas; Leppänen, Elli; Lilius, Tuomas; Kalso, Eija; Mikladal, Bjo̷rn; Varjos, Ilkka; Koskinen, Jari; Laurila, Tomi
Contributor organization: INDIVIDRUG - Individualized Drug Therapy
HUSLAB
Medicum
Department of Clinical Pharmacology
Department of Pharmacology
University of Helsinki
Helsinki University Hospital Area
HUS Emergency Medicine and Services
HUS Perioperative, Intensive Care and Pain Medicine
Eija Kalso / Principal Investigator
Department of Diagnostics and Therapeutics
Clinicum
Anestesiologian yksikkö
Date: 2021-05-04
Language: eng
Number of pages: 7
Belongs to series: ACS Omega
ISSN: 2470-1343
DOI: https://doi.org/10.1021/acsomega.1c00773
URI: http://hdl.handle.net/10138/332455
Abstract: Disposable single-use electrochemical sensor strips were used for quantitative detection of small concentrations of morphine in untreated capillary whole blood. Single-walled carbon nanotube (SWCNT) networks were fabricated on a polymer substrate to produce flexible, reproducible sensor strips with integrated reference and counter electrodes, compatible with industrial-scale processes. A thin Nafion coating was used on top of the sensors to enable direct electrochemical detection in whole blood. These sensors were shown to detect clinically relevant concentrations of morphine both in buffer and in whole blood samples. Small 38 mu L finger-prick blood samples were spiked with 2 mu L of morphine solution of several concentrations and measured without precipitation of proteins or any other further pretreatment. A linear range of 0.5-10 mu M was achieved in both matrices and a detection limit of 0.48 mu M in buffer. In addition, to demonstrate the applicability of the sensor in a point-of-care device, single-determination measurements were done with capillary samples from three subjects. An average recovery of 60% was found, suggesting that the sensor only measures the free, unbound fraction of the drug. An interference study with other opioids and possible interferents showed the selectivity of the sensor. This study clearly indicates that these Nafion/SWCNT sensor strips show great promise as a point-of-care rapid test for morphine in blood.
Subject: 3121 General medicine, internal medicine and other clinical medicine
WALLED CARBON NANOTUBES
PROTEIN-BINDING
HUMAN-SERUM
CODEINE
ELECTRODE
NANOPARTICLES
PHARMACOKINETICS
OXYCODONE
TRAMADOL
SAMPLES
Peer reviewed: Yes
Rights: cc_by
Usage restriction: openAccess
Self-archived version: publishedVersion


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