Inactivation of the gene encoding the cationic antimicrobial peptide resistance factor MprF increases biofilm formation but reduces invasiveness of Listeria monocytogenes

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Nowak , J , Visnovsky , S B , Cruz , C D , Fletcher , G C , van Vliet , A H M , Hedderley , D , Butler , R , Flint , S , Palmer , J & Pitman , A R 2021 , ' Inactivation of the gene encoding the cationic antimicrobial peptide resistance factor MprF increases biofilm formation but reduces invasiveness of Listeria monocytogenes ' , Journal of Applied Microbiology , vol. 130 , no. 2 , pp. 464-477 . https://doi.org/10.1111/jam.14790

Title: Inactivation of the gene encoding the cationic antimicrobial peptide resistance factor MprF increases biofilm formation but reduces invasiveness of Listeria monocytogenes
Author: Nowak, J.; Visnovsky, S. B.; Cruz, C. D.; Fletcher, G. C.; van Vliet, A. H. M.; Hedderley, D.; Butler, R.; Flint, S.; Palmer, J.; Pitman, A. R.
Contributor organization: Bioactivity Screening Group
Division of Pharmaceutical Biosciences
Drug Research Program
Date: 2021-02
Language: eng
Number of pages: 14
Belongs to series: Journal of Applied Microbiology
ISSN: 1364-5072
DOI: https://doi.org/10.1111/jam.14790
URI: http://hdl.handle.net/10138/332489
Abstract: Aims To understand the genetics involved in surface attachment and biofilm formation ofListeria monocytogenes. Methods and Results Anin vitroscreen of a Himar1 transposon library ofL. monocytogenesstrain 15G01 identified three transposants that produced significantly different biofilm levels when compared to the wild-type strain; two mutants exhibited enhanced biofilm formation and one produced less biofilm biomass than the wild-type. The mutant 15G01mprF::Himar1, which had a transposon insertion in themprFgene, was selected for further analysis. The mutant produced a more densely populated biofilm on solid surfaces such as stainless steel and polystyrene, as determined using scanning electron and light microscopy. The 15G01mprF::Himar1 mutant remained viable in biofilms, but showed an increase in sensitivity to the cationic antimicrobial gallidermin. The mutant also displayed reduced invasiveness in CaCo-2 intestinal cells, suggesting virulence properties are compromised by the inactivation ofmprF. Conclusions Biofilm formation and gallidermin resistance ofL. monocytogenesis influenced bymprF, but this trait is associated with a compromise in invasiveness. Significance and Impact of the Study The presence of pathogenic microorganisms in the food processing environment can cause a significant problem, especially when these microorganisms are established as biofilms. This study shows that the inactivation of themprFgene results in enhanced biofilm formation and abiotic surface attachment ofL. monocytogenes.
Subject: biofilm
food safety
Listeria monocytogenes
mprF
mutagenesis library
LISTERIA-MONOCYTOGENES
EXTRACELLULAR DNA
STRAINS
GROWTH
VIRULENCE
CONTAMINATION
PROTEIN
VIRR
ACID
11832 Microbiology and virology
Peer reviewed: Yes
Rights: unspecified
Usage restriction: openAccess
Self-archived version: acceptedVersion


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