Chromosome Level Assembly of the Comma Butterfly (Polygonia c-album)

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Celorio-Mancera , M D L P , Rastas , P , Steward , R A , Nylin , S & Wheat , C W 2021 , ' Chromosome Level Assembly of the Comma Butterfly (Polygonia c-album) ' , Genome Biology and Evolution , vol. 13 , no. 5 , 054 .

Titel: Chromosome Level Assembly of the Comma Butterfly (Polygonia c-album)
Författare: Celorio-Mancera, Maria de la Paz; Rastas, Pasi; Steward, Rachel A.; Nylin, Soren; Wheat, Christopher W.
Upphovmannens organisation: Institute of Biotechnology
Datum: 2021-05
Språk: eng
Sidantal: 6
Tillhör serie: Genome Biology and Evolution
ISSN: 1759-6653
Permanenta länken (URI):
Abstrakt: The comma butterfly (Polygonia c-album, Nymphalidae, Lepidoptera) is a model insect species, most notably in the study of phenotypic plasticity and plant-insect coevolutionary interactions. In order to facilitate the integration of genomic tools with a diverse body of ecological and evolutionary research, we assembled the genome of a Swedish comma using 10X sequencing, scaffolding with matepair data, genome polishing, and assignment to linkage groups using a high-density linkage map. The resulting genome is 373 Mb in size, with a scaffold N50 of 11.7 Mb and contig N50 of 11,2Mb. The genome contained 90.1% of single-copy Lepidopteran orthologs in a BUSCO analysis of 5,286 genes. A total of 21,004 gene-models were annotated on the genome using RNA-Seq data from larval and adult tissue in combination with proteins from the Arthropoda database, resulting in a high-quality annotation for which functional annotations were generated. We further documented the quality of the chromosomal assembly via synteny assessment with Melitaea cinxia. The resulting annotated, chromosome-level genome will provide an important resource for investigating coevolutionary dynamics and comparative analyses in Lepidoptera.
Subject: linkage map
butterfly genome
quantitative annotation assessment
comparative genomics
Polygonia c-album
1181 Ecology, evolutionary biology
Referentgranskad: Ja
Licens: cc_by_nc
Användningsbegränsning: openAccess
Parallelpublicerad version: publishedVersion

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