Inamdar , K , Tsai , F-C , Dibsy , R , de Poret , A , Manzi , J , Merida , P , Muller , R , Lappalainen , P , Roingeard , P , Mak , J , Bassereau , P , Favard , C & Muriaux , D 2021 , ' Full assembly of HIV-1 particles requires assistance of the membrane curvature factor IRSp53 ' , eLife , vol. 10 , 67321 . https://doi.org/10.7554/eLife.67321
Title: | Full assembly of HIV-1 particles requires assistance of the membrane curvature factor IRSp53 |
Author: | Inamdar, Kaushik; Tsai, Feng-Ching; Dibsy, Rayane; de Poret, Aurore; Manzi, John; Merida, Peggy; Muller, Remi; Lappalainen, Pekka; Roingeard, Philippe; Mak, Johnson; Bassereau, Patricia; Favard, Cyril; Muriaux, Delphine |
Contributor organization: | Institute of Biotechnology Biosciences |
Date: | 2021-06-11 |
Language: | eng |
Number of pages: | 26 |
Belongs to series: | eLife |
ISSN: | 2050-084X |
DOI: | https://doi.org/10.7554/eLife.67321 |
URI: | http://hdl.handle.net/10138/332610 |
Abstract: | During HIV-1 particle formation, the requisite plasma membrane curvature is thought to be solely driven by the retroviral Gag protein. Here, we reveal that the cellular I-BAR protein IRSp53 is required for the progression of HIV-1 membrane curvature to complete particle assembly. siRNA-mediated knockdown of IRSp53 gene expression induces a decrease in viral particle production and a viral bud arrest at half completion. Single-molecule localization microscopy at the cell plasma membrane shows a preferential localization of IRSp53 around HIV-1 Gag assembly sites. In addition, we observe the presence of IRSp53 in purified HIV-1 particles. Finally, HIV-1 Gag protein preferentially localizes to curved membranes induced by IRSp53 I-BAR domain on giant unilamellar vesicles. Overall, our data reveal a strong interplay between IRSp53 I-BAR and Gag at membranes during virus assembly. This highlights IRSp53 as a crucial host factor in HIV-1 membrane curvature and its requirement for full HIV-1 particle assembly. |
Subject: |
COLOCALIZATION ANALYSIS
VIRUS DOMAIN CDC42 INHIBITION MECHANISM PROTEINS RELEASE PATHWAY BINDING 1182 Biochemistry, cell and molecular biology |
Peer reviewed: | Yes |
Rights: | cc_by |
Usage restriction: | openAccess |
Self-archived version: | publishedVersion |
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