Transcriptional study reveals a potential leptin-dependent gene regulatory network in zebrafish brain

Show full item record



Permalink

http://hdl.handle.net/10138/332699

Citation

Ahi , E P , Tsakoumis , E , Brunel , M & Schmitz , M 2021 , ' Transcriptional study reveals a potential leptin-dependent gene regulatory network in zebrafish brain ' , Fish Physiology & Biochemistry , vol. 47 , pp. 1283-1298 . https://doi.org/10.1007/s10695-021-00967-0

Title: Transcriptional study reveals a potential leptin-dependent gene regulatory network in zebrafish brain
Author: Ahi, Ehsan Pashay; Tsakoumis, Emmanouil; Brunel, Mathilde; Schmitz, Monika
Contributor: University of Helsinki, Organismal and Evolutionary Biology Research Programme
Date: 2021-07-08
Language: eng
Number of pages: 16
Belongs to series: Fish Physiology & Biochemistry
ISSN: 0920-1742
URI: http://hdl.handle.net/10138/332699
Abstract: The signal mediated by leptin hormone and its receptor is a major regulator of body weight, food intake and metabolism. In mammals and many teleost fish species, leptin has an anorexigenic role and inhibits food intake by influencing the appetite centres in the hypothalamus. However, the regulatory connections between leptin and downstream genes mediating its appetite-regulating effects are still not fully explored in teleost fish. In this study, we used a loss of function leptin receptor zebrafish mutant and real-time quantitative PCR to assess brain expression patterns of several previously identified anorexigenic genes downstream of leptin signal under different feeding conditions (normal feeding, 7-day fasting, 2 and 6-h refeeding). These downstream factors include members of cart genes, crhb and gnrh2, as well as selected genes co-expressed with them based on a zebrafish co-expression database. Here, we found a potential gene expression network (GRN) comprising the abovementioned genes by a stepwise approach of identifying co-expression modules and predicting their upstream regulators. Among the transcription factors (TFs) predicted as potential upstream regulators of this GRN, we found expression pattern of sp3a to be correlated with transcriptional changes of the downstream gene network. Interestingly, the expression and transcriptional activity of Sp3 orthologous gene in mammals have already been implicated to be under the influence of leptin signal. These findings suggest a potentially conserved regulatory connection between leptin and sp3a, which is predicted to act as a transcriptional driver of a downstream gene network in the zebrafish brain.
Subject: Leptin receptor
Zebrafish
Gene expression
Gene regulatory network
Feeding
Brain
CONCENTRATING HORMONE GENES
MESSENGER-RNA EXPRESSION
NEUROPEPTIDE-Y
FOOD-INTAKE
COCAINE
EGR-1
GROWTH
CART
SP1
REPRESSION
1182 Biochemistry, cell and molecular biology
Rights:


Files in this item

Total number of downloads: Loading...

Files Size Format View
Ahi2021_Article_TranscriptionalStudyRevealsAPo.pdf 1.264Mb PDF View/Open

This item appears in the following Collection(s)

Show full item record