Partitioning and Spatial Distribution of Drugs in Ocular Surface Tissues

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Balla , A , Auriola , S , Grey , A C , Demarais , N J , Valtari , A , Heikkinen , E M , Toropainen , E , Urtti , A , Vellonen , K-S & Ruponen , M 2021 , ' Partitioning and Spatial Distribution of Drugs in Ocular Surface Tissues ' , Pharmaceutics , vol. 13 , no. 5 , 658 . https://doi.org/10.3390/pharmaceutics13050658

Title: Partitioning and Spatial Distribution of Drugs in Ocular Surface Tissues
Author: Balla, Anusha; Auriola, Seppo; Grey, Angus C.; Demarais, Nicholas J.; Valtari, Annika; Heikkinen, Emma M.; Toropainen, Elisa; Urtti, Arto; Vellonen, Kati-Sisko; Ruponen, Marika
Contributor organization: Drug Research Program
Drug Delivery
Drug Delivery Unit
Division of Pharmaceutical Biosciences
Date: 2021-05
Language: eng
Number of pages: 15
Belongs to series: Pharmaceutics
ISSN: 1999-4923
DOI: https://doi.org/10.3390/pharmaceutics13050658
URI: http://hdl.handle.net/10138/332741
Abstract: Ocular drug absorption after eye drop instillation has been widely studied, but partitioning phenomena and spatial drug distribution are poorly understood. We investigated partitioning of seven beta-blocking drugs in corneal epithelium, corneal stroma, including endothelium and conjunctiva, using isolated porcine tissues and cultured human corneal epithelial cells. The chosen beta-blocking drugs had a wide range (-1.76-0.79) of n-octanol/buffer solution distribution coefficients at pH 7.4 (Log D-7.4). In addition, the ocular surface distribution of three beta-blocking drugs was determined by matrix-assisted laser desorption/ionization imaging mass spectrometry (MALDI-IMS) after their simultaneous application in an eye drop to the rabbits in vivo. Studies with isolated porcine corneas revealed that the distribution coefficient (K-p) between the corneal epithelium and donor solution showed a positive relationship and good correlation with Log D-7.4 and about a 50-fold range of K-p values (0.1-5). On the contrary, K-p between corneal stroma and epithelium showed an inverse (negative) relationship and correlation with Log D-7.4 based on a seven-fold range of K-p values. In vitro corneal cell uptake showed a high correlation with the ex vivo corneal epithelium/donor K-p values. Partitioning of the drugs into the porcine conjunctiva also showed a positive relationship with lipophilicity, but the range of K-p values was less than with the corneal epithelium. MALDI-IMS allowed simultaneous detection of three compounds in the cornea, showed data in line with other experiments, and revealed uneven spatial drug distribution in the cornea. Our data indicate the importance of lipophilicity in defining the corneal pharmacokinetics and the K-p values are a useful building block in the kinetic simulation models for topical ocular drug administration.
Subject: cornea
conjunctiva
drug absorption
permeation
beta-blocking agent
MALDI-IMS
SYSTEMIC ABSORPTION
OPHTHALMIC EMULSION
PIGMENTED RABBIT
CORNEAL
PERMEABILITY
CONJUNCTIVAL
PENETRATION
PHARMACOKINETICS
PILOCARPINE
CYCLOSPORINE
317 Pharmacy
Peer reviewed: Yes
Rights: cc_by
Usage restriction: openAccess
Self-archived version: publishedVersion


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