Controlled Monofunctionalization of Molecular Spherical Nucleic Acids on a Buckminster Fullerene Core

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dc.contributor.author Gulumkar, Vijay
dc.contributor.author Äärelä, Antti
dc.contributor.author Moisio, Olli
dc.contributor.author Rahkila, Jani
dc.contributor.author Tähtinen, Ville
dc.contributor.author Leimu, Laura
dc.contributor.author Korsoff, Niko
dc.contributor.author Korhonen, Heidi
dc.contributor.author Poijärvi-Virta, Päivi
dc.contributor.author Mikkola, Satu
dc.contributor.author Nesati, Victor
dc.contributor.author Vuorimaa-Laukkanen, Elina
dc.contributor.author Viitala, Tapani
dc.contributor.author Yliperttula, Marjo
dc.contributor.author Roivainen, Anne
dc.contributor.author Virta, Pasi
dc.date.accessioned 2021-08-02T11:59:01Z
dc.date.available 2021-08-02T11:59:01Z
dc.date.issued 2021-06-16
dc.identifier.citation Gulumkar , V , Äärelä , A , Moisio , O , Rahkila , J , Tähtinen , V , Leimu , L , Korsoff , N , Korhonen , H , Poijärvi-Virta , P , Mikkola , S , Nesati , V , Vuorimaa-Laukkanen , E , Viitala , T , Yliperttula , M , Roivainen , A & Virta , P 2021 , ' Controlled Monofunctionalization of Molecular Spherical Nucleic Acids on a Buckminster Fullerene Core ' , Bioconjugate Chemistry , vol. 32 , no. 6 , pp. 1130-1138 . https://doi.org/10.1021/acs.bioconjchem.1c00187
dc.identifier.other PURE: 167122935
dc.identifier.other PURE UUID: d9b377b9-f0d5-492b-b7c7-9bb656ed3dd9
dc.identifier.other WOS: 000664301900010
dc.identifier.other ORCID: /0000-0001-9074-9450/work/97969385
dc.identifier.uri http://hdl.handle.net/10138/332748
dc.description.abstract An azide-functionalized 12-armed Buckminster fullerene has been monosubstituted in organic media with a substoichiometric amount of cyclooctyne-modified oligonucleo-tides. Exposing the intermediate products then to the same reaction (i. e., strain-promoted alkyne-azide cycloaddition, SPAAC) with an excess of slightly different oligonucleotide constituents in an aqueous medium yields molecularly defined monofunctionalized spherical nucleic acids (SNAs). This procedure offers a controlled synthesis scheme in which one oligonucleotide arm can be functionalized with labels or other conjugate groups (1,4,7,10-tetraazacyclododecane-1,4,7,10-tetraacetic acid, DOTA, and Alexa-488 demonstrated), whereas the rest of the 11 arms can be left unmodified or modified by other conjugate groups in order to decorate the SNAs' outer sphere. Extra attention has been paid to the homogeneity and authenticity of the C60-azide scaffold used for the assembly of full-armed SNAs. en
dc.format.extent 9
dc.language.iso eng
dc.relation.ispartof Bioconjugate Chemistry
dc.rights cc_by
dc.rights.uri info:eu-repo/semantics/openAccess
dc.subject CELLULAR UPTAKE
dc.subject CLICK CHEMISTRY
dc.subject MECHANISM
dc.subject AZIDES
dc.subject 317 Pharmacy
dc.title Controlled Monofunctionalization of Molecular Spherical Nucleic Acids on a Buckminster Fullerene Core en
dc.type Article
dc.contributor.organization Divisions of Faculty of Pharmacy
dc.contributor.organization Division of Pharmaceutical Chemistry and Technology
dc.contributor.organization Division of Pharmaceutical Biosciences
dc.contributor.organization Drug Research Program
dc.contributor.organization Pharmaceutical biophysics group
dc.contributor.organization Biopharmaceutics Group
dc.description.reviewstatus Peer reviewed
dc.relation.doi https://doi.org/10.1021/acs.bioconjchem.1c00187
dc.relation.issn 1043-1802
dc.rights.accesslevel openAccess
dc.type.version publishedVersion

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