N1-acetylspermidine is a determinant of hair follicle stem cell fate

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Allmeroth, K, Kim, C S, Annibal, A, Pouikli, A, Koester, J, Derisbourg, M J, Chacon-Martinez, C A, Latza, C, Antebi, A, Tessarz, P, Wickström, S A & Denzel, M S 2021, ' N1-acetylspermidine is a determinant of hair follicle stem cell fate ', Journal of Cell Science, vol. 134, no. 9, 252767 . https://doi.org/10.1242/jcs.252767

Title: N1-acetylspermidine is a determinant of hair follicle stem cell fate
Author: Allmeroth, Kira; Kim, Christine S.; Annibal, Andrea; Pouikli, Andromachi; Koester, Janis; Derisbourg, Maxime J.; Chacon-Martinez, Carlos Andres; Latza, Christian; Antebi, Adam; Tessarz, Peter; Wickström, Sara A.; Denzel, Martin S.
Contributor organization: Department of Biochemistry and Developmental Biology
STEMM - Stem Cells and Metabolism Research Program
Helsinki Institute of Life Science HiLIFE
Faculty of Medicine
Date: 2021-05
Language: eng
Number of pages: 17
Belongs to series: Journal of Cell Science
ISSN: 0021-9533
DOI: https://doi.org/10.1242/jcs.252767
URI: http://hdl.handle.net/10138/332799
Abstract: Stem cell differentiation is accompanied by increased mRNA translation. The rate of protein biosynthesis is influenced by the polyamines putrescine, spermidine and spermine, which are essential for cell growth and stem cell maintenance. However, the role of polyamines as endogenous effectors of stem cell fate and whether they act through translational control remains obscure. Here, we investigate the function of polyamines in stem cell fate decisions using hair follicle stem cell (HFSC) organoids. Compared to progenitor cells, HFSCs showed lower translation rates, correlating with reduced polyamine levels. Surprisingly, overall polyamine depletion decreased translation but did not affect cell fate. In contrast, specific depletion of natural polyamines mediated by spermidine/spermine N1-acetyltransferase (SSAT; also known as SAT1) activation did not reduce translation but enhanced stemness. These results suggest a translation-independent role of polyamines in cell fate regulation. Indeed, we identified N1-acetylspermidine as a determinant of cell fate that acted through increasing self-renewal, and observed elevated N1-acetylspermidine levels upon depilation-mediated HFSC proliferation and differentiation in vivo. Overall, this study delineates the diverse routes of polyamine metabolism-mediated regulation of stem cell fate decisions. This article has an associated First Person interview with the first author of the paper.
Subject: Hair follicle stem cells
Polyamines
Cell fate
mRNA translation
SPERMIDINE-SPERMINE N-1-ACETYLTRANSFERASE
ORNITHINE-DECARBOXYLASE
PROTEIN-SYNTHESIS
OSTEOGENIC DIFFERENTIATION
PROMOTES TRANSLATION
SELF-RENEWAL
POLYAMINES
CYCLE
IDENTIFICATION
INHIBITION
1182 Biochemistry, cell and molecular biology
Peer reviewed: Yes
Rights: cc_by
Usage restriction: openAccess
Self-archived version: publishedVersion


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