Minimum information guideline for spectrophotometric and fluorometric methods to assess biofilm formation in microplates

Show full item record



Permalink

http://hdl.handle.net/10138/332927

Citation

Allkja , J , Bjarnsholt , T , Coenye , T , Cos , P , Fallarero , A , Harrison , J J , Lopes , S P , Oliver , A , Pereira , M O , Ramage , G , Shirtliff , M E , Stoodley , P , Webb , J S , Zaat , S A J , Goeres , D M & Azevedo , N F 2020 , ' Minimum information guideline for spectrophotometric and fluorometric methods to assess biofilm formation in microplates ' , Biofilm , vol. 2 , 100010 . https://doi.org/10.1016/j.bioflm.2019.100010

Title: Minimum information guideline for spectrophotometric and fluorometric methods to assess biofilm formation in microplates
Author: Allkja, Jontana; Bjarnsholt, Thomas; Coenye, Tom; Cos, Paul; Fallarero, Adyary; Harrison, Joe J.; Lopes, Susana P.; Oliver, Antonio; Pereira, Maria Olivia; Ramage, Gordon; Shirtliff, Mark E.; Stoodley, Paul; Webb, Jeremy S.; Zaat, Sebastian A. J.; Goeres, Darla M.; Azevedo, Nuno Filipe
Contributor: University of Helsinki, Divisions of Faculty of Pharmacy
Date: 2020-12
Language: eng
Number of pages: 8
Belongs to series: Biofilm
ISSN: 2590-2075
URI: http://hdl.handle.net/10138/332927
Abstract: The lack of reproducibility of published studies is one of the major issues facing the scientific community, and the field of biofilm microbiology has been no exception. One effective strategy against this multifaceted problem is the use of minimum information guidelines. This strategy provides a guide for authors and reviewers on the necessary information that a manuscript should include for the experiments in a study to be clearly interpreted and independently reproduced. As a result of several discussions between international groups working in the area of biofilms, we present a guideline for the spectrophotometric and fluorometric assessment of biofilm formation in microplates. This guideline has been divided into 5 main sections, each presenting a comprehensive set of recommendations. The intention of the minimum information guideline is to improve the quality of scientific communication that will augment interlaboratory reproducibility in biofilm microplate assays.
Subject: Biofilm
Reproducibility
Guidelines
Microplate
Spectrophotometry
Fluorometry
PSEUDOMONAS-AERUGINOSA BIOFILM
STAPHYLOCOCCUS-AUREUS
METABOLIC-ACTIVITY
CANDIDA-ALBICANS
VIABILITY ASSAYS
CRYSTAL VIOLET
QUANTIFICATION
SUSCEPTIBILITY
ADHESION
BIOMASS
317 Pharmacy
Rights:


Files in this item

Total number of downloads: Loading...

Files Size Format View
1_s2.0_S2590207519300103_main.pdf 672.4Kb PDF View/Open

This item appears in the following Collection(s)

Show full item record