The structure and biosynthesis of heinamides A1-A3 and B1-B5, antifungal members of the laxaphycin lipopeptide family

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Heinilä , L M P , Fewer , D P , Jokela , J K , Wahlsten , M , Ouyang , X , Permi , P , Jortikka , A & Sivonen , K 2021 , ' The structure and biosynthesis of heinamides A1-A3 and B1-B5, antifungal members of the laxaphycin lipopeptide family ' , Organic & Biomolecular Chemistry , vol. 19 , no. 25 , pp. 5577-5588 . https://doi.org/10.1039/d1ob00772f

Title: The structure and biosynthesis of heinamides A1-A3 and B1-B5, antifungal members of the laxaphycin lipopeptide family
Author: Heinilä, Lassi Matti Petteri; Fewer, David Peter; Jokela, Jouni Kalevi; Wahlsten, Matti; Ouyang, Xiaodan; Permi, Perttu; Jortikka, Anna; Sivonen, Kaarina
Contributor: University of Helsinki, Cyanobacteria research
University of Helsinki, Department of Microbiology
University of Helsinki, Faculty of Agriculture and Forestry
University of Helsinki, Department of Microbiology
University of Helsinki, Department of Microbiology
University of Helsinki, Department of Microbiology
University of Helsinki, Department of Microbiology
Date: 2021-07-07
Language: eng
Number of pages: 12
Belongs to series: Organic & Biomolecular Chemistry
ISSN: 1477-0520
URI: http://hdl.handle.net/10138/333168
Abstract: Laxaphycins are a family of cyclic lipopeptides with synergistic antifungal and antiproliferative activities. They are produced by multiple cyanobacterial genera and comprise two sets of structurally unrelated 11- and 12-residue macrocyclic lipopeptides. Here, we report the discovery of new antifungal laxaphycins from Nostoc sp. UHCC 0702, which we name heinamides, through antimicrobial bioactivity screening. We characterized the chemical structures of eight heinamide structural variants A1-A3 and B1-B5. These variants contain the rare non-proteinogenic amino acids 3-hydroxy-4-methylproline, 4-hydroxyproline, 3-hydroxy-d-leucine, dehydrobutyrine, 5-hydroxyl beta-amino octanoic acid, and O-carbamoyl-homoserine. We obtained an 8.6-Mb complete genome sequence from Nostoc sp. UHCC 0702 and identified the 93 kb heinamide biosynthetic gene cluster. The structurally distinct heinamides A1-A3 and B1-B5 variants are synthesized using an unusual branching biosynthetic pathway. The heinamide biosynthetic pathway also encodes several enzymes that supply non-proteinogenic amino acids to the heinamide synthetase. Through heterologous expression, we showed that (2S,4R)-4-hydroxy-l-proline is supplied through the action of a novel enzyme LxaN, which hydroxylates l-proline. 11- and 12-residue heinamides have the characteristic synergistic activity of laxaphycins against Aspergillus flavus FBCC 2467. Structural and genetic information of heinamides may prove useful in future discovery of natural products and drug development.
Subject: ALGA ANABAENA-LAXA
GENE-CLUSTER
BIOCHEMICAL-CHARACTERIZATION
CYCLIC LIPOPEPTIDES
CELL-PROLIFERATION
PEPTIDES
CYANOBACTERIA
INHIBITORS
PRODUCT
HYDROXYPROLINE
116 Chemical sciences
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