Cell influx and contractile actomyosin force drive mammary bud growth and invagination

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Trela , E , Lan , Q , Myllymäki , S-M , Villeneuve , C , Lindström , R , Kumar , V , Wickström , S A & Mikkola , M L 2021 , ' Cell influx and contractile actomyosin force drive mammary bud growth and invagination ' , Journal of Cell Biology , vol. 220 , no. 8 , 202008062 . https://doi.org/10.1083/jcb.202008062

Title: Cell influx and contractile actomyosin force drive mammary bud growth and invagination
Author: Trela, Ewelina; Lan, Qiang; Myllymäki, Satu-Marja; Villeneuve, Clémentine; Lindström, Riitta; Kumar, Vinod; Wickström, Sara A.; Mikkola, Marja L.
Contributor: University of Helsinki, Institute of Biotechnology
University of Helsinki, Institute of Biotechnology
University of Helsinki, Institute of Biotechnology
University of Helsinki, STEMM - Stem Cells and Metabolism Research Program
University of Helsinki, Institute of Biotechnology
University of Helsinki, Institute of Biotechnology
University of Helsinki, STEMM - Stem Cells and Metabolism Research Program
University of Helsinki, Institute of Biotechnology
Date: 2021-08-02
Language: eng
Number of pages: 26
Belongs to series: Journal of Cell Biology
ISSN: 0021-9525
URI: http://hdl.handle.net/10138/333258
Abstract: The mammary gland develops from the surface ectoderm during embryogenesis and proceeds through morphological phases defined as placode, hillock, bud, and bulb stages followed by branching morphogenesis. During this early morphogenesis, the mammary bud undergoes an invagination process where the thickened bud initially protrudes above the surface epithelium and then transforms to a bulb and sinks into the underlying mesenchyme. The signaling pathways regulating the early morphogenetic steps have been identified to some extent, but the underlying cellular mechanisms remain ill defined. Here, we use 3D and 4D confocal microscopy to show that the early growth of the mammary rudiment is accomplished by migration-driven cell influx, with minor contributions of cell hypertrophy and proliferation. We delineate a hitherto undescribed invagination mechanism driven by thin, elongated keratinocytes-ring cells-that form a contractile rim around the mammary bud and likely exert force via the actomyosin network. Furthermore, we show that conditional deletion of nonmuscle myosin IIA (NMIIA) impairs invagination, resulting in abnormal mammary bud shape.
Subject: DIFFERENTIATION
LINE
MIGRATION
MORPHOGENESIS
MORPHOLOGY
MOUSE
POLARITY
PROLIFERATION
ROLES
SONIC-HEDGEHOG
1182 Biochemistry, cell and molecular biology
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