Avela , H F & Siren , H 2020 , ' Advances in analytical tools and current statistical methods used in ultra-high-performance liquid chromatography-mass spectrometry of glycero-, glycerophospho- and sphingolipids ' , International Journal of Mass Spectrometry , vol. 457 , 116408 . https://doi.org/10.1016/j.ijms.2020.116408
Title: | Advances in analytical tools and current statistical methods used in ultra-high-performance liquid chromatography-mass spectrometry of glycero-, glycerophospho- and sphingolipids |
Author: | Avela, Henri F.; Siren, Heli |
Contributor organization: | Department of Chemistry |
Date: | 2020-11 |
Language: | eng |
Number of pages: | 18 |
Belongs to series: | International Journal of Mass Spectrometry |
ISSN: | 1387-3806 |
DOI: | https://doi.org/10.1016/j.ijms.2020.116408 |
URI: | http://hdl.handle.net/10138/333288 |
Abstract: | The review concentrates on the properties of analytical and statistical ultrahigh-performance liquid chromatographic (UHPLC) - mass spectrometric (MS) methods suitable for glycero-, glycerophospho- and sphingolipids in lipidomics published between the years 2017 2019. Trends and fluctuations of conventional and nano-UHPLC methods with MS and tandem MS detection were observed in context of analysis conditions and tools used for data-analysis. Whereas general workflow characteristics are agreed upon, more details related to the chromatographic methodology (i.e. stationary and mobile phase conditions) need evidently agreements. Lipid quantitation relies upon isotope-labelled standards in targeted analyses and fully standardless algorithm-based untargeted analyses. Furthermore, a wide spectrum of setups have shown potential for the elucidation of complex and large datasets by minimizing the risks of systematic misinterpretation like false positives. This kind of evaluation was shown to have increased importance and usage for cross-validation and data-analysis. (C) 2020 Elsevier B.V. All rights reserved. |
Subject: |
Lipidomics
Mass spectrometry Ultrahigh performance liquid chromatography Nano-liquid chromatography Chemometrics Statistical methods Multicomponent analysis FIELD-FLOW FRACTIONATION SUPERCRITICAL-FLUID CHROMATOGRAPHY PRESSURE CHEMICAL-IONIZATION FALSE DISCOVERY RATE ESI-QTOF-MS/MS ELECTROSPRAY-IONIZATION LIPIDOMIC ANALYSIS FATTY-ACID QUANTIFICATION EXTRACTION 116 Chemical sciences |
Peer reviewed: | Yes |
Rights: | cc_by_nc_nd |
Usage restriction: | openAccess |
Self-archived version: | acceptedVersion |
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