Heterologous Expression of the Leuconostoc Bacteriocin Leucocin C in Probiotic Yeast Saccharomyces boulardii

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Ran , L , Wan , X , Takala , T & Saris , P 2021 , ' Heterologous Expression of the Leuconostoc Bacteriocin Leucocin C in Probiotic Yeast Saccharomyces boulardii ' , Probiotics and Antimicrobial Proteins , vol. 13 , pp. 229-237 . https://doi.org/10.1007/s12602-020-09676-1

Title: Heterologous Expression of the Leuconostoc Bacteriocin Leucocin C in Probiotic Yeast Saccharomyces boulardii
Author: Ran, Li; Wan, Xing; Takala, Timo; Saris, Per
Contributor: University of Helsinki, Department of Microbiology
University of Helsinki, Department of Microbiology
University of Helsinki, Department of Microbiology
University of Helsinki, Department of Microbiology
Date: 2021-02
Language: eng
Belongs to series: Probiotics and Antimicrobial Proteins
ISSN: 1867-1306
URI: http://hdl.handle.net/10138/333469
Abstract: The yeastSaccharomyces boulardiiis well known for its probiotic effects such as treating or preventing gastrointestinal diseases. Due to its ability to survive in stomach and intestine,S. boulardiicould be applied as a vehicle for producing and delivering bioactive substances of interest to human gut. In this study, we cloned the genelecCencoding the antilisterial peptide leucocin C from lactic acid bacteriumLeuconostoc carnosuminS. boulardii. The constructedS. boulardiistrain secreted a peptide, which had molecular weight corresponding to leucocin C in SDS-PAGE. The peptide band inhibitedListeria monocytogenesin gel overlay assay. Likewise, concentratedS. boulardiiculture supernatant inhibited the growth ofL. monocytogenes. The growth profile and acid tolerance of the leucocin C secretingS. boulardiiwere similar as those of the strain carrying the empty vector. We further demonstrated that the cells of the leucocin C producingS. boulardiiefficiently killedL. monocytogenes, also without antibiotic selection pressure. These results showed that antilisterial activity could be added to the arsenal of probiotic activities ofS. boulardii, demonstrating its potential as a carrier for therapeutics delivery.
Subject: 416 Food Science
Probiotic yeast
Saccharomyces boulardii
Bacteriocin
Leucocin C
Heterologous expression
LACTIC-ACID BACTERIA
SACCHAROMYCES-BOULARDII
LISTERIA-MONOCYTOGENES
CEREVISIAE
TRANSLOCATION
INTERFERES
INHIBITION
PROMOTERS
EFFICACY
DIARRHEA
DISEASE
11832 Microbiology and virology
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