Sea-ice bacteria, Halomonas sp. 363 and Paracoccus sp. 392, produce multiple types of poly-3-hydroxyalkaonoic acid (PHA) storage polymers at low temperature

Show full item record



Permalink

http://hdl.handle.net/10138/333562

Citation

Eronen-Rasimus , E L , Hultman , J , Hai , T , Pessi , I S , Collins , E , Wright , S , Laine , P , Viitamäki , S , Lyra , C , Thomas , D N , Golyshin , P , Luhtanen , A-M , Kuosa , H & Kaartokallio , H 2021 , ' Sea-ice bacteria, Halomonas sp. 363 and Paracoccus sp. 392, produce multiple types of poly-3-hydroxyalkaonoic acid (PHA) storage polymers at low temperature ' , Applied and Environmental Microbiology , vol. 87 , no. 17 , 00929-21 . https://doi.org/10.1128/AEM.00929-21

Title: Sea-ice bacteria, Halomonas sp. 363 and Paracoccus sp. 392, produce multiple types of poly-3-hydroxyalkaonoic acid (PHA) storage polymers at low temperature
Author: Eronen-Rasimus, Eeva Liisa; Hultman, Jenni; Hai, T; Pessi, Igor S; Collins, Eric; Wright, S; Laine, Pia; Viitamäki, Sirja; Lyra, Christina; Thomas, David Neville; Golyshin, Peter; Luhtanen, Anne-Mari; Kuosa, Harri; Kaartokallio, Hermanni
Contributor: University of Helsinki, Department of Microbiology
University of Helsinki, Department of Microbiology
University of Helsinki, Department of Microbiology
University of Helsinki, Institute of Biotechnology
University of Helsinki, Arctic Microbial Ecology
University of Helsinki, Fungal Genetics and Biotechnology
University of Helsinki, Ecosystems and Environment Research Programme
University of Helsinki, Molecular Principles of Viruses
Date: 2021-06-23
Language: eng
Number of pages: 12
Belongs to series: Applied and Environmental Microbiology
ISSN: 0099-2240
URI: http://hdl.handle.net/10138/333562
Abstract: Poly-3-hydroxyalkanoic acids (PHAs) are bacterial storage polymers commonly used in bioplastic production. Halophilic bacteria are industrially interesting organisms as their salinity tolerance and psychrophilic nature lowers sterility requirements and subsequent production costs. We investigated the PHA synthesis in two bacterial strains, Halomonas sp. 363 and Paracoccus sp. 392, isolated from Southern Ocean sea ice and elucidated the related PHA biopolymer accumulation and composition with various approaches, such as transcriptomics, microscopy and chromatography. We show that both bacterial strains produce PHAs at 4 °C when the availability of nitrogen and/or oxygen limited growth. The genome of Halomonas sp. 363 encoded three phaC synthase genes and transcribed genes along three PHA pathways (I–III), whereas Paracoccus sp. 392 carry only one phaC gene and transcribed genes along one pathway (I). Thus Halomonas sp. 363 has versatile repertoire of phaC genes and pathways enabling production of both short- and medium chain length PHA products.
Subject: 11832 Microbiology and virology
Rights:


Files in this item

Total number of downloads: Loading...

Files Size Format View
AEM.00929_21.pdf 1.388Mb PDF View/Open

This item appears in the following Collection(s)

Show full item record