VC1 catalyzes a key step in the biosynthesis of vicine from GTP in faba bean

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Bjornsdotter , E , Nadzieja , M , Chang , W , Escobar-Herrera , L , Mancinotti , D , Angra , D , Khazaei , H , Crocoll , C , Vandenberg , A , Stoddard , F L , O’Sullivan , D M , Stougaard , J , Schulman , A H , Andersen , S U & Geu-Flores , F 2021 , ' VC1 catalyzes a key step in the biosynthesis of vicine from GTP in faba bean ' , Nature plants , vol. 7 , pp. 923–931 . https://doi.org/10.1101/2020.02.26.966523

Title: VC1 catalyzes a key step in the biosynthesis of vicine from GTP in faba bean
Author: Bjornsdotter, Emilie; Nadzieja, Marcin; Chang, Wei; Escobar-Herrera, Leandro; Mancinotti, Davide; Angra, Deepti; Khazaei, Hamid; Crocoll, Christoph; Vandenberg, Albert; Stoddard, Frederick L.; O’Sullivan, Donal M.; Stougaard, Jens; Schulman, Alan H.; Andersen, Stig Uggerhoj; Geu-Flores, Fernando
Contributor: University of Helsinki, Institute of Biotechnology
University of Helsinki, Faculty of Agriculture and Forestry
University of Helsinki, Department of Agricultural Sciences
University of Helsinki, Institute of Biotechnology
Date: 2021-07-05
Language: eng
Belongs to series: Nature plants
ISSN: 2055-026X
URI: http://hdl.handle.net/10138/333654
Abstract: Faba bean is a widely adapted and high-yielding legume cultivated for its protein-rich seeds. However, the seeds accumulate the anti-nutritional pyrimidine glucosides vicine and convicine, which can cause haemolytic anaemia (favism) in the 400 million individuals genetically predisposed by a deficiency in glucose-6-phosphate dehydrogenase. Here, we identify the first enzyme associated with vicine and convicine biosynthesis, which we name VC1. We show that VC1 co-locates with the major QTL for vicine and convicine content and that the expression of VC1 correlates highly with vicine content across tissues. We also show that low-vicine varieties express a version of VC1 carrying a small, frame-shift insertion, and that overexpression of wild-type VC1 leads to an increase in vicine levels. VC1 encodes a functional GTP cyclohydrolase II, an enzyme normally involved in riboflavin biosynthesis from the purine GTP. Through feeding studies, we demonstrate that GTP is a precursor of vicine both in faba bean and in the distantly related plant bitter gourd. Our results reveal an unexpected biosynthetic origin for vicine and convicine and pave the way for the development of faba bean cultivars that are free from these anti-nutrients, providing a safe and sustainable source of dietary protein.
Subject: 11831 Plant biology
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