Skeletal muscle proteomes reveal downregulation of mitochondrial proteins in transition from prediabetes into type 2 diabetes

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Öhman , T , Teppo , J , Datta , N , Mäkinen , S , Varjosalo , M & Koistinen , H A 2021 , ' Skeletal muscle proteomes reveal downregulation of mitochondrial proteins in transition from prediabetes into type 2 diabetes ' , iScience , vol. 24 , no. 7 , 102712 . https://doi.org/10.1016/j.isci.2021.102712

Title: Skeletal muscle proteomes reveal downregulation of mitochondrial proteins in transition from prediabetes into type 2 diabetes
Author: Öhman, Tiina; Teppo, Jaakko; Datta, Neeta; Mäkinen, Selina; Varjosalo, Markku; Koistinen, Heikki A.
Contributor: University of Helsinki, Molecular Systems Biology
University of Helsinki, Division of Pharmaceutical Chemistry and Technology
University of Helsinki, HUS Internal Medicine and Rehabilitation
University of Helsinki, Institute of Biotechnology
University of Helsinki, HUS Internal Medicine and Rehabilitation
Date: 2021-07-23
Language: eng
Number of pages: 22
Belongs to series: iScience
ISSN: 2589-0042
URI: http://hdl.handle.net/10138/333655
Abstract: Skeletal muscle insulin resistance is a central defect in the pathogenesis of type 2 diabetes (T2D). Here, we analyzed skeletal muscle proteome in 148 vastus lateralis muscle biopsies obtained from men covering all glucose tolerance phenotypes: normal, impaired fasting glucose (IFG), impaired glucose tolerance (IGT) and T2D. Skeletal muscle proteome was analyzed by a sequential window acquisition of all theoretical mass spectra (SWATH-MS) proteomics technique. Our data indicate a downregulation in several proteins involved inmitochondrial electron transport or respiratory chain complex assembly already in IFG and IGT-muscles, with most profound decreases observed in T2D. Additional phosphoproteomic analysis reveals altered phosphorylation in several signaling pathways in IFG, IGT, and T2D muscles, including those regulating glucose metabolic processes, and the structure of muscle cells. These data reveal several alterations present in skeletalmuscle already in prediabetes and highlight impairedmitochondrial energy metabolism in the trajectory from prediabetes into T2D.
Subject: INSULIN-RESISTANCE
GLUCOSE-TRANSPORT
HSUV3P HELICASE
ATP SYNTHASE
GENE
ENRICHMENT
PHOSPHORYLATION
PHOSPHOPROTEOME
DYSLIPIDEMIA
METABOLISM
1182 Biochemistry, cell and molecular biology
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