Anti-Fouling Effects of Saponin-Containing Crude Extracts from Tropical Indo-Pacific Sea Cucumbers

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http://hdl.handle.net/10138/333657

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Kamyab , E , Goebeler , N , Kellermann , M Y , Rohde , S , Reverter , M , Striebel , M & Schupp , P J 2020 , ' Anti-Fouling Effects of Saponin-Containing Crude Extracts from Tropical Indo-Pacific Sea Cucumbers ' , Marine Drugs , vol. 18 , no. 4 , 181 . https://doi.org/10.3390/md18040181

Title: Anti-Fouling Effects of Saponin-Containing Crude Extracts from Tropical Indo-Pacific Sea Cucumbers
Author: Kamyab, Elham; Goebeler, Norman; Kellermann, Matthias Y.; Rohde, Sven; Reverter, Miriam; Striebel, Maren; Schupp, Peter J.
Date: 2020-04
Language: eng
Number of pages: 19
Belongs to series: Marine Drugs
ISSN: 1660-3397
URI: http://hdl.handle.net/10138/333657
Abstract: Sea cucumbers are bottom dwelling invertebrates, which are mostly found on subtropical and tropical sea grass beds, sandy reef flats, or reef slopes. Although constantly exposed to fouling communities in these habitats, many species are surprisingly free of invertebrate epibionts and microfouling algae such as diatoms. In our study, we investigated the anti-fouling (AF) activities of different crude extracts of tropical Indo-Pacific sea cucumber species against the fouling diatom Cylindrotheca closterium. Nine sea cucumber species from three genera (i.e., Holothuria, Bohadschia, Actinopyga) were selected and extracted to assess their AF activities. To verify whether the sea cucumber characteristic triterpene glycosides were responsible for the observed potent AF activities, we tested purified fractions enriched in saponins isolated from Bohadschia argus, representing one of the most active anti-fouling extracts. Saponins were quantified by vanillin-sulfuric acid colorimetric assays and identified by LC-MS and LC-MS/MS analyses. We were able to demonstrate that AF activities in sea cucumber extracts were species-specific, and growth inhibition as well as attachment of the diatom to surfaces is dependent on the saponin concentration (i.e., Actinopyga contained the highest quantities), as well as on the molecular composition and structure of the present saponins (i.e., Bivittoside D derivative was the most bioactive compound). In conclusion, the here performed AF assay represents a promising and fast method for selecting the most promising bioactive organism as well as for identifying novel compounds with potent AF activities for the discovery of potentially novel pharmacologically active natural products.
Subject: holothurian
diatom
anti-fouling compounds
marine natural products
saponins
triterpene glycosides
mass spectrometry
MARINE NATURAL-PRODUCTS
TRITERPENE GLYCOSIDES
ANTIFOULING ACTIVITY
STRUCTURAL ELUCIDATION
CHEMICAL DEFENSE
BIVITTOSIDE-A
HOLOTHUROIDEA
GROWTH
OLIGOGLYCOSIDES
ECHINODERMATA
317 Pharmacy
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