Anti-Fouling Effects of Saponin-Containing Crude Extracts from Tropical Indo-Pacific Sea Cucumbers

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dc.contributor.author Kamyab, Elham
dc.contributor.author Goebeler, Norman
dc.contributor.author Kellermann, Matthias Y.
dc.contributor.author Rohde, Sven
dc.contributor.author Reverter, Miriam
dc.contributor.author Striebel, Maren
dc.contributor.author Schupp, Peter J.
dc.date.accessioned 2021-08-26T10:03:02Z
dc.date.available 2021-08-26T10:03:02Z
dc.date.issued 2020-04
dc.identifier.citation Kamyab , E , Goebeler , N , Kellermann , M Y , Rohde , S , Reverter , M , Striebel , M & Schupp , P J 2020 , ' Anti-Fouling Effects of Saponin-Containing Crude Extracts from Tropical Indo-Pacific Sea Cucumbers ' , Marine Drugs , vol. 18 , no. 4 , 181 . https://doi.org/10.3390/md18040181
dc.identifier.other PURE: 159272453
dc.identifier.other PURE UUID: 17566140-c0bc-4552-942f-b54b74d97f29
dc.identifier.other WOS: 000533909300018
dc.identifier.uri http://hdl.handle.net/10138/333657
dc.description.abstract Sea cucumbers are bottom dwelling invertebrates, which are mostly found on subtropical and tropical sea grass beds, sandy reef flats, or reef slopes. Although constantly exposed to fouling communities in these habitats, many species are surprisingly free of invertebrate epibionts and microfouling algae such as diatoms. In our study, we investigated the anti-fouling (AF) activities of different crude extracts of tropical Indo-Pacific sea cucumber species against the fouling diatom Cylindrotheca closterium. Nine sea cucumber species from three genera (i.e., Holothuria, Bohadschia, Actinopyga) were selected and extracted to assess their AF activities. To verify whether the sea cucumber characteristic triterpene glycosides were responsible for the observed potent AF activities, we tested purified fractions enriched in saponins isolated from Bohadschia argus, representing one of the most active anti-fouling extracts. Saponins were quantified by vanillin-sulfuric acid colorimetric assays and identified by LC-MS and LC-MS/MS analyses. We were able to demonstrate that AF activities in sea cucumber extracts were species-specific, and growth inhibition as well as attachment of the diatom to surfaces is dependent on the saponin concentration (i.e., Actinopyga contained the highest quantities), as well as on the molecular composition and structure of the present saponins (i.e., Bivittoside D derivative was the most bioactive compound). In conclusion, the here performed AF assay represents a promising and fast method for selecting the most promising bioactive organism as well as for identifying novel compounds with potent AF activities for the discovery of potentially novel pharmacologically active natural products. en
dc.format.extent 19
dc.language.iso eng
dc.relation.ispartof Marine Drugs
dc.rights cc_by
dc.rights.uri info:eu-repo/semantics/openAccess
dc.subject holothurian
dc.subject diatom
dc.subject anti-fouling compounds
dc.subject marine natural products
dc.subject saponins
dc.subject triterpene glycosides
dc.subject mass spectrometry
dc.subject MARINE NATURAL-PRODUCTS
dc.subject TRITERPENE GLYCOSIDES
dc.subject ANTIFOULING ACTIVITY
dc.subject STRUCTURAL ELUCIDATION
dc.subject CHEMICAL DEFENSE
dc.subject BIVITTOSIDE-A
dc.subject HOLOTHUROIDEA
dc.subject GROWTH
dc.subject OLIGOGLYCOSIDES
dc.subject ECHINODERMATA
dc.subject 317 Pharmacy
dc.title Anti-Fouling Effects of Saponin-Containing Crude Extracts from Tropical Indo-Pacific Sea Cucumbers en
dc.type Article
dc.contributor.organization Tvärminne Zoological Station
dc.description.reviewstatus Peer reviewed
dc.relation.doi https://doi.org/10.3390/md18040181
dc.relation.issn 1660-3397
dc.rights.accesslevel openAccess
dc.type.version publishedVersion

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