RTN4B interacting protein FAM134C promotes ER membrane curvature and has a functional role in autophagy

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dc.contributor University of Helsinki, Institute of Biotechnology en
dc.contributor University of Helsinki, Molecular and Integrative Biosciences Research Programme en
dc.contributor University of Helsinki, Institute of Biotechnology en
dc.contributor University of Helsinki, Institute of Biotechnology en
dc.contributor University of Helsinki, Institute of Biotechnology en
dc.contributor University of Helsinki, Institute of Biotechnology en
dc.contributor University of Helsinki, Institute of Biotechnology en
dc.contributor University of Helsinki, Institute of Biotechnology en
dc.contributor University of Helsinki, Institute of Biotechnology en
dc.contributor University of Helsinki, Institute of Biotechnology en
dc.contributor University of Helsinki, Institute of Biotechnology en
dc.contributor.author Kumar, Darshan
dc.contributor.author Lak, Behnam
dc.contributor.author Suntio, Taina
dc.contributor.author Vihinen, Helena
dc.contributor.author Belevich, Ilya
dc.contributor.author Viita, Tiina
dc.contributor.author Xiaonan, Liu
dc.contributor.author Vartiainen, Aki
dc.contributor.author Vartiainen, Maria
dc.contributor.author Varjosalo, Markku
dc.contributor.author Jokitalo, Eija
dc.date.accessioned 2021-08-26T11:51:02Z
dc.date.available 2021-08-26T11:51:02Z
dc.date.issued 2021-06-01
dc.identifier.citation Kumar , D , Lak , B , Suntio , T , Vihinen , H , Belevich , I , Viita , T , Xiaonan , L , Vartiainen , A , Vartiainen , M , Varjosalo , M & Jokitalo , E 2021 , ' RTN4B interacting protein FAM134C promotes ER membrane curvature and has a functional role in autophagy ' , Molecular Biology of the Cell , vol. 32 , no. 12 , pp. 1158-1170 . https://doi.org/10.1091/mbc.E20-06-0409 en
dc.identifier.issn 1059-1524
dc.identifier.other PURE: 166761389
dc.identifier.other PURE UUID: 4bb48e33-4bbe-4bf0-98fe-e8329041485d
dc.identifier.other WOS: 000656487500004
dc.identifier.other ORCID: /0000-0003-2190-4909/work/97266174
dc.identifier.other ORCID: /0000-0003-3862-9237/work/97266952
dc.identifier.other ORCID: /0000-0002-1340-9732/work/97268689
dc.identifier.other ORCID: /0000-0002-2017-0475/work/97269189
dc.identifier.other ORCID: /0000-0002-4159-6934/work/97269504
dc.identifier.uri http://hdl.handle.net/10138/333663
dc.description.abstract The endoplasmic reticulum (ER) is composed of a controlled ratio of sheets and tubules, which are maintained by several proteins with multiple functions. Reticulons (RTNs), especially RTN4, and DP1Nop1p family members are known to induce ER membrane curvature. RTN4B is the main RTN4 isoform expressed in nonneuronal cells. In this study, we identified FAM134C as a RTN4B interacting protein in mammalian, nonneuronal cells. FAM134C localized specifically to the ER tubules and sheet edges. Ultrastructural analysis revealed that overexpression of FAM134C induced the formation of unbranched, long tubules or dense globular structures composed of heavily branched narrow tubules. In both cases, tubules were nonmotile. ER tubulation was dependent on the reticulon homology domain (RHD) close to the N-terminus. FAM134C plays a role in the autophagy pathway as its level elevated significantly upon amino acid starvation but not during ER stress. Moreover, FAM134C depletion reduced the number and size of autophagic structures and the amount of ER as a cargo within autophagic structures under starvation conditions. Dominant-negative expression of FAM134C forms with mutated RHD or LC3 interacting region also led to a reduced number of autophagic structures. Our results suggest that FAM134C provides a link between regulation of ER architecture and ER turnover by promoting ER tubulation required for subsequent ER fragmentation and engulfment into autophagosomes. en
dc.format.extent 13
dc.language.iso eng
dc.relation.ispartof Molecular Biology of the Cell
dc.rights en
dc.subject ENDOPLASMIC-RETICULUM en
dc.subject CELLS en
dc.subject VISUALIZATION en
dc.subject MORPHOLOGY en
dc.subject NOGO en
dc.subject WEB en
dc.subject 1182 Biochemistry, cell and molecular biology en
dc.title RTN4B interacting protein FAM134C promotes ER membrane curvature and has a functional role in autophagy en
dc.type Article
dc.description.version Peer reviewed
dc.identifier.doi https://doi.org/10.1091/mbc.E20-06-0409
dc.type.uri info:eu-repo/semantics/other
dc.type.uri info:eu-repo/semantics/publishedVersion
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