Blood Leukocyte Signaling Pathways as Predictors of Severity of Acute Pancreatitis

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dc.contributor.author Turunen, Antti
dc.contributor.author Kuuliala, Antti
dc.contributor.author Mustonen, Harri
dc.contributor.author Puolakkainen, Pauli
dc.contributor.author Kylänpää, Leena
dc.contributor.author Kuuliala, Krista
dc.date.accessioned 2021-09-01T10:03:01Z
dc.date.available 2021-09-01T10:03:01Z
dc.date.issued 2021
dc.identifier.citation Turunen , A , Kuuliala , A , Mustonen , H , Puolakkainen , P , Kylänpää , L & Kuuliala , K 2021 , ' Blood Leukocyte Signaling Pathways as Predictors of Severity of Acute Pancreatitis ' , Pancreas , vol. 50 , no. 5 , pp. 710-718 . https://doi.org/10.1097/MPA.0000000000001832
dc.identifier.other PURE: 168065564
dc.identifier.other PURE UUID: 4ab11af8-9c4f-43ea-a141-092da84e17ef
dc.identifier.other WOS: 000667268200014
dc.identifier.other ORCID: /0000-0001-5632-6796/work/99331223
dc.identifier.uri http://hdl.handle.net/10138/333899
dc.description.abstract Objectives Clinical practice lacks biomarkers to predict the severity of acute pancreatitis (AP). We studied if intracellular signaling of circulating leukocytes could predict persistent organ dysfunction (OD) and secondary infections in AP. Methods A venous blood sample was taken from 174 patients with AP 72 hours or less from onset of symptoms and 31 healthy controls. Phosphorylation levels (p) of appropriately stimulated signal transducer and activator of transcription 1 (STAT1), STAT6, nuclear factor-kappa B (NF-kappa B), Akt, and nonstimulated STAT3 in monocytes, neutrophils, and lymphocytes was measured using phosphospecific flow cytometry. Results The patients showed higher pSTAT3 and lower pSTAT1, pSTAT6, pNF-kappa B, and pAkt than healthy controls. pSTAT3 in all leukocyte subtypes studied increased, and pSTAT1 in monocytes and T cells decreased in an AP severity-wise manner. In patients without OD at sampling, high pSTAT3 in monocytes and T lymphocytes were associated with development of persistent OD. In patients with OD, low interleukin-4-stimulated pSTAT6 in monocytes and neutrophils and Escherichia coli-stimulated pNF-kappa B in neutrophils predicted OD persistence. High pSTAT3 in monocytes, CD8(+) T cells, and neutrophils; low pSTAT1 in monocytes and T cells; and low pNF-kappa B in lymphocytes predicted secondary infections. Conclusions Leukocyte STAT3, STAT1, STAT6, and NF-kappa Beta phosphorylations are potential predictors of AP severity. en
dc.format.extent 9
dc.language.iso eng
dc.relation.ispartof Pancreas
dc.rights cc_by
dc.rights.uri info:eu-repo/semantics/openAccess
dc.subject pancreatitis
dc.subject prognosis
dc.subject blood
dc.subject leukocytes
dc.subject signal transduction
dc.subject flow cytometry
dc.subject NF-KAPPA-B
dc.subject ORGAN FAILURE
dc.subject INFLAMMATION
dc.subject DYSFUNCTION
dc.subject SEPSIS
dc.subject STAT3
dc.subject ASSOCIATION
dc.subject ACTIVATION
dc.subject TOLERANCE
dc.subject THERAPIES
dc.subject 3121 General medicine, internal medicine and other clinical medicine
dc.subject 3126 Surgery, anesthesiology, intensive care, radiology
dc.title Blood Leukocyte Signaling Pathways as Predictors of Severity of Acute Pancreatitis en
dc.type Article
dc.contributor.organization HUS Abdominal Center
dc.contributor.organization Medicum
dc.contributor.organization Department of Bacteriology and Immunology
dc.contributor.organization University of Helsinki
dc.contributor.organization Helsinki University Hospital Area
dc.contributor.organization University Management
dc.contributor.organization Clinicum
dc.contributor.organization Department of Surgery
dc.contributor.organization II kirurgian klinikka
dc.contributor.organization Teachers' Academy
dc.contributor.organization Pauli Puolakkainen / Principal Investigator
dc.description.reviewstatus Peer reviewed
dc.relation.doi https://doi.org/10.1097/MPA.0000000000001832
dc.relation.issn 0885-3177
dc.rights.accesslevel openAccess
dc.type.version publishedVersion

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