Neuropilin-2 Is Associated With Increased Hepatoblastoma Cell Viability and Motility

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Eloranta , K , Nousiainen , R , Cairo , S , Pakarinen , M P , Wilson , D B , Pihlajoki , M & Heikinheimo , M 2021 , ' Neuropilin-2 Is Associated With Increased Hepatoblastoma Cell Viability and Motility ' , Frontiers in pediatrics , vol. 9 , 660482 . https://doi.org/10.3389/fped.2021.660482

Title: Neuropilin-2 Is Associated With Increased Hepatoblastoma Cell Viability and Motility
Author: Eloranta, Katja; Nousiainen, Ruth; Cairo, Stefano; Pakarinen, Mikko P.; Wilson, David B.; Pihlajoki, Marjut; Heikinheimo, Markku
Contributor organization: HUS Children and Adolescents
Children's Hospital
Lastenkirurgian yksikkö
Medicum
Clinicum
Helsinki One Health (HOH)
Developmental and tumor biology research group
Date: 2021-06-22
Language: eng
Number of pages: 10
Belongs to series: Frontiers in pediatrics
ISSN: 2296-2360
DOI: https://doi.org/10.3389/fped.2021.660482
URI: http://hdl.handle.net/10138/333931
Abstract: The neuropilins NRP1 and NRP2 are multifunctional glycoproteins that have been implicated in several cancer-related processes including cell survival, migration, and invasion in various tumor types. Here, we examine the role of neuropilins in hepatoblastoma (HB), the most common pediatric liver malignancy. Using a combination of immunohistochemistry, RNA analysis and western blotting, we observed high level expression of NRP1 and NRP2 in 19 of 20 HB specimens and in a majority of human HB cell lines (HUH6 and five cell lines established from patient-derived xenografts) studied but not in normal hepatocytes. Silencing of NRP2 expression in HUH6 and HB-282 HB cells resulted in decreased cell viability, impaired cytoskeleton remodeling, and reduced cell motility, suggesting that NRP2 contributes to the malignant phenotype. We propose that neuropilins warrant further investigation as biomarkers of HB and potential therapeutic targets.
Subject: neuropilin
hepatoblastoma
pediatric cancer
cell viability
migration
liver
CANCER-CELLS
POOR-PROGNOSIS
BETA-CATENIN
TUMOR-GROWTH
EXPRESSION
INVASION
SUPPRESSES
CARCINOMA
MIGRATION
PROLIFERATION
3123 Gynaecology and paediatrics
Peer reviewed: Yes
Rights: cc_by
Usage restriction: openAccess
Self-archived version: publishedVersion


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