Populus PtERF85 Balances Xylem Cell Expansion and Secondary Cell Wall Formation in Hybrid Aspen

Show full item record



Permalink

http://hdl.handle.net/10138/334185

Citation

Seyfferth , C , Wessels , B A , Vahala , J , Kangasjarvi , J , Delhomme , N , Hvidsten , T R , Tuominen , H & Lundberg-Felten , J 2021 , ' Populus PtERF85 Balances Xylem Cell Expansion and Secondary Cell Wall Formation in Hybrid Aspen ' , Cells , vol. 10 , no. 8 , 1971 . https://doi.org/10.3390/cells10081971

Title: Populus PtERF85 Balances Xylem Cell Expansion and Secondary Cell Wall Formation in Hybrid Aspen
Author: Seyfferth, Carolin; Wessels, Bernard A.; Vahala, Jorma; Kangasjarvi, Jaakko; Delhomme, Nicolas; Hvidsten, Torgeir R.; Tuominen, Hannele; Lundberg-Felten, Judith
Contributor: University of Helsinki, Swedish University of Agricultural Sciences
University of Helsinki, Faculty of Biological and Environmental Sciences
University of Helsinki, Plant Biology
Date: 2021-08
Language: eng
Number of pages: 19
Belongs to series: Cells
ISSN: 2073-4409
URI: http://hdl.handle.net/10138/334185
Abstract: Secondary growth relies on precise and specialized transcriptional networks that determine cell division, differentiation, and maturation of xylem cells. We identified a novel role for the ethylene-induced Populus Ethylene Response Factor PtERF85 (Potri.015G023200) in balancing xylem cell expansion and secondary cell wall (SCW) formation in hybrid aspen (Populus tremula x tremuloides). Expression of PtERF85 is high in phloem and cambium cells and during the expansion of xylem cells, while it is low in maturing xylem tissue. Extending PtERF85 expression into SCW forming zones of woody tissues through ectopic expression reduced wood density and SCW thickness of xylem fibers but increased fiber diameter. Xylem transcriptomes from the transgenic trees revealed transcriptional induction of genes involved in cell expansion, translation, and growth. The expression of genes associated with plant vascular development and the biosynthesis of SCW chemical components such as xylan and lignin, was down-regulated in the transgenic trees. Our results suggest that PtERF85 activates genes related to xylem cell expansion, while preventing transcriptional activation of genes related to SCW formation. The importance of precise spatial expression of PtERF85 during wood development together with the observed phenotypes in response to ectopic PtERF85 expression suggests that PtERF85 contributes to the transition of fiber cells from elongation to secondary cell wall deposition.
Subject: cell wall thickness
ERF85 (CRF4)
lignin
ribosome biogenesis
wood development
xylem expansion
CYTOKININ RESPONSE FACTORS
ARABIDOPSIS
BIOSYNTHESIS
EXPRESSION
GROWTH
CELLULOSE
DYNAMICS
ETHYLENE
PATHWAY
1182 Biochemistry, cell and molecular biology
Rights:


Files in this item

Total number of downloads: Loading...

Files Size Format View
cells_10_01971_v4.pdf 6.075Mb PDF View/Open

This item appears in the following Collection(s)

Show full item record