Populus PtERF85 Balances Xylem Cell Expansion and Secondary Cell Wall Formation in Hybrid Aspen

Show full item record




Seyfferth , C , Wessels , B A , Vahala , J , Kangasjarvi , J , Delhomme , N , Hvidsten , T R , Tuominen , H & Lundberg-Felten , J 2021 , ' Populus PtERF85 Balances Xylem Cell Expansion and Secondary Cell Wall Formation in Hybrid Aspen ' , Cells , vol. 10 , no. 8 , 1971 . https://doi.org/10.3390/cells10081971

Title: Populus PtERF85 Balances Xylem Cell Expansion and Secondary Cell Wall Formation in Hybrid Aspen
Author: Seyfferth, Carolin; Wessels, Bernard A.; Vahala, Jorma; Kangasjarvi, Jaakko; Delhomme, Nicolas; Hvidsten, Torgeir R.; Tuominen, Hannele; Lundberg-Felten, Judith
Contributor organization: Faculty of Biological and Environmental Sciences
Organismal and Evolutionary Biology Research Programme
Plant Biology
Viikki Plant Science Centre (ViPS)
Plant ROS-Signalling
Date: 2021-08
Language: eng
Number of pages: 19
Belongs to series: Cells
ISSN: 2073-4409
DOI: https://doi.org/10.3390/cells10081971
URI: http://hdl.handle.net/10138/334185
Abstract: Secondary growth relies on precise and specialized transcriptional networks that determine cell division, differentiation, and maturation of xylem cells. We identified a novel role for the ethylene-induced Populus Ethylene Response Factor PtERF85 (Potri.015G023200) in balancing xylem cell expansion and secondary cell wall (SCW) formation in hybrid aspen (Populus tremula x tremuloides). Expression of PtERF85 is high in phloem and cambium cells and during the expansion of xylem cells, while it is low in maturing xylem tissue. Extending PtERF85 expression into SCW forming zones of woody tissues through ectopic expression reduced wood density and SCW thickness of xylem fibers but increased fiber diameter. Xylem transcriptomes from the transgenic trees revealed transcriptional induction of genes involved in cell expansion, translation, and growth. The expression of genes associated with plant vascular development and the biosynthesis of SCW chemical components such as xylan and lignin, was down-regulated in the transgenic trees. Our results suggest that PtERF85 activates genes related to xylem cell expansion, while preventing transcriptional activation of genes related to SCW formation. The importance of precise spatial expression of PtERF85 during wood development together with the observed phenotypes in response to ectopic PtERF85 expression suggests that PtERF85 contributes to the transition of fiber cells from elongation to secondary cell wall deposition.
Subject: cell wall thickness
ERF85 (CRF4)
ribosome biogenesis
wood development
xylem expansion
1182 Biochemistry, cell and molecular biology
Peer reviewed: Yes
Rights: cc_by
Usage restriction: openAccess
Self-archived version: publishedVersion

Files in this item

Total number of downloads: Loading...

Files Size Format View
cells_10_01971_v4.pdf 6.075Mb PDF View/Open

This item appears in the following Collection(s)

Show full item record