Bacteriophages fEV-1 and fD1 Infect Yersinia pestis

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Skurnik , M , Jaakkola , S , Mattinen , L , von Ossowski , L , Nawaz , A , Pajunen , M & Happonen , L J 2021 , ' Bacteriophages fEV-1 and fD1 Infect Yersinia pestis ' , Viruses (Basel) , vol. 13 , no. 7 , 1384 . https://doi.org/10.3390/v13071384

Title: Bacteriophages fEV-1 and fD1 Infect Yersinia pestis
Author: Skurnik, Mikael; Jaakkola, Salla; Mattinen, Laura; von Ossowski, Lotta; Nawaz, Ayesha; Pajunen, Maria; Happonen, Lotta J.
Contributor organization: Research Programs Unit
HUMI - Human Microbiome Research
Department of Bacteriology and Immunology
Helsinki One Health (HOH)
HUSLAB
Mikael Skurnik / Principal Investigator
Medicum
Biosciences
Faculty of Medicine
University of Helsinki
Glycoscience Group
Date: 2021-07
Language: eng
Number of pages: 21
Belongs to series: Viruses (Basel)
ISSN: 1999-4915
DOI: https://doi.org/10.3390/v13071384
URI: http://hdl.handle.net/10138/334232
Abstract: Bacteriophages vB_YpeM_fEV-1 (fEV-1) and vB_YpeM_fD1 (fD1) were isolated from incoming sewage water samples in Turku, Finland, using Yersinia pestis strains EV76 and KIM D27 as enrichment hosts, respectively. Genomic analysis and transmission electron microscopy established that fEV-1 is a novel type of dwarf myovirus, while fD1 is a T4-like myovirus. The genome sizes are 38 and 167 kb, respectively. To date, the morphology and genome sequences of some dwarf myoviruses have been described; however, a proteome characterization such as the one presented here, has currently been lacking for this group of viruses. Notably, fEV-1 is the first dwarf myovirus described for Y. pestis. The host range of fEV-1 was restricted strictly to Y. pestis strains, while that of fD1 also included other members of Enterobacterales such as Escherichia coli and Yersinia pseudotuberculosis. In this study, we present the life cycles, genomes, and proteomes of two Yersinia myoviruses, fEV-1 and fD1.
Subject: bacteriophage
Yersinia pestis
dwarf myovirus
myovirus
genome
proteome
GENOME SEQUENCE
O-ANTIGEN
ENTEROCOLITICA O-8
MASS-SPECTROMETRY
VIRULENCE
LIPOPOLYSACCHARIDE
PSEUDOTUBERCULOSIS
IDENTIFICATION
PLATFORM
ARTEMIS
3111 Biomedicine
11832 Microbiology and virology
Peer reviewed: Yes
Rights: cc_by
Usage restriction: openAccess
Self-archived version: publishedVersion


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