Host-pathogen adhesion as the basis of innovative diagnostics for emerging pathogens

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van Belkum , A , Almeida , C , Bardiaux , B , Barrass , S V , Butcher , S J , Çaykara , T , Chowdhury , S , Datar , R , Eastwood , I , Goldman , A , Goyal , M , Izadi-Pruneyre , N , Jacobsen , T , Johnson , P H , Kempf , V A J , Kiessling , A , Bueno , J L , Malik , A , Malmström , J , Meuskens , I , Milner , P A , Nilges , M , Pamme , N , Peyman , S A , Rodrigues , L R , Rodriguez-Mateos , P , Sande , M G , Silva , C J , Stehle , T , Thibau , A , Vaca , D J & Linke , D 2021 , ' Host-pathogen adhesion as the basis of innovative diagnostics for emerging pathogens ' , Diagnostics , vol. 11 , no. 7 , 1259 . https://doi.org/10.3390/diagnostics11071259

Title: Host-pathogen adhesion as the basis of innovative diagnostics for emerging pathogens
Author: van Belkum, Alex; Almeida, Carina; Bardiaux, Benjamin; Barrass, Sarah V.; Butcher, Sarah J.; Çaykara, Tuğçe; Chowdhury, Sounak; Datar, Rucha; Eastwood, Ian; Goldman, Adrian; Goyal, Manisha; Izadi-Pruneyre, Nadia; Jacobsen, Theis; Johnson, Pirjo H.; Kempf, Volkhard A.J.; Kiessling, Andreas; Bueno, Juan Leva; Malik, Anchal; Malmström, Johan; Meuskens, Ina; Milner, Paul A.; Nilges, Michael; Pamme, Nicole; Peyman, Sally A.; Rodrigues, Ligia R.; Rodriguez-Mateos, Pablo; Sande, Maria G.; Silva, Carla Joana; Stehle, Thilo; Thibau, Arno; Vaca, Diana J.; Linke, Dirk
Contributor organization: University of Helsinki
Doctoral Programme in Integrative Life Science
Molecular and Integrative Biosciences Research Programme
Institute of Biotechnology
Biosciences
Macromolecular structure and function
Doctoral Programme in Microbiology and Biotechnology
Biochemistry and Biotechnology
Doctoral Programme Brain & Mind
Date: 2021-07
Language: eng
Number of pages: 22
Belongs to series: Diagnostics
ISSN: 2075-4418
DOI: https://doi.org/10.3390/diagnostics11071259
URI: http://hdl.handle.net/10138/334319
Abstract: Infectious diseases are an existential health threat, potentiated by emerging and re-emerging viruses and increasing bacterial antibiotic resistance. Targeted treatment of infectious diseases requires precision diagnostics, especially in cases where broad-range therapeutics such as antibiotics fail. There is thus an increasing need for new approaches to develop sensitive and specific in vitro diagnostic (IVD) tests. Basic science and translational research are needed to identify key microbial molecules as diagnostic targets, to identify relevant host counterparts, and to use this knowledge in developing or improving IVD. In this regard, an overlooked feature is the capacity of pathogens to adhere specifically to host cells and tissues. The molecular entities relevant for pathogen-surface interaction are the so-called adhesins. Adhesins vary from protein compounds to (poly-)saccharides or lipid structures that interact with eukaryotic host cell matrix molecules and receptors. Such interactions co-define the specificity and sensitivity of a diagnostic test. Currently, adhesin-receptor binding is typically used in the pre-analytical phase of IVD tests, focusing on pathogen enrichment. Further exploration of adhesin-ligand interaction, supported by present high-throughput "omics" technologies, might stimulate a new generation of broadly applicable pathogen detection and characterization tools. This review describes recent results of novel structure-defining technologies allowing for detailed molecular analysis of adhesins, their receptors and complexes. Since the host ligands evolve slowly, the corresponding adhesin interaction is under selective pressure to maintain a constant receptor binding domain. IVD should exploit such conserved binding sites and, in particular, use the human ligand to enrich the pathogen. We provide an inventory of methods based on adhesion factors and pathogen attachment mechanisms, which can also be of relevance to currently emerging pathogens, including SARS-CoV-2, the causative agent of COVID-19.
Description: Funding Information: The authors gratefully thank J?rgen Berger and Katharina Hipp (both Max Planck-Institute for Developmental Biology, T?bingen, Germany) for the electron microscopy dis-played in Figure 1.This research was funded by the European Union?s Horizon 2020 research and innovation program in a project named Viral and Bacterial Adhesin Network Training (ViBrANT) under Marie Sk?odowska-Curie Grant Agreement No. 765042. A.G. also acknowledges support from the BBSRC (grant number BB/M021610/1). Publisher Copyright: © 2021 by the authors. Licensee MDPI, Basel, Switzerland.
Subject: adhesin
receptor
infectious diseases
diagnostics
STAPHYLOCOCCUS-AUREUS
ESCHERICHIA-COLI
YERSINIA-ENTEROCOLITICA
BARTONELLA-HENSELAE
INFECTIOUS-DISEASES
SENSITIVE DETECTION
MATRIX COMPONENTS
MASS-SPECTROMETRY
STRUCTURAL BASIS
INFLUENZA-VIRUS
3121 General medicine, internal medicine and other clinical medicine
Peer reviewed: Yes
Rights: cc_by
Usage restriction: openAccess
Self-archived version: publishedVersion


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