Fecal Bacteria Implicated in Biofilm Production Are Enriched and Associate to Gastrointestinal Symptoms in Patients With APECED - A Pilot Study

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dc.contributor.author Hetemäki, Iivo
dc.contributor.author Jian, Ching
dc.contributor.author Laakso, Saila Marita
dc.contributor.author Mäkitie, Outi
dc.contributor.author Pajari, Anne-Maria
dc.contributor.author Vos de, Willem Meindert
dc.contributor.author Arstila, Petteri
dc.contributor.author Salonen, Anne
dc.date.accessioned 2021-09-14T07:24:01Z
dc.date.available 2021-09-14T07:24:01Z
dc.date.issued 2021-07
dc.identifier.citation Hetemäki , I , Jian , C , Laakso , S M , Mäkitie , O , Pajari , A-M , Vos de , W M , Arstila , P & Salonen , A 2021 , ' Fecal Bacteria Implicated in Biofilm Production Are Enriched and Associate to Gastrointestinal Symptoms in Patients With APECED - A Pilot Study ' , Frontiers in Immunology , vol. 12 , 668219 . https://doi.org/10.3389/fimmu.2021.668219
dc.identifier.other PURE: 166806327
dc.identifier.other PURE UUID: 2b35ab90-720a-4a6f-b8c4-2dd05535bb92
dc.identifier.other WOS: 000682794900001
dc.identifier.other ORCID: /0000-0002-6248-4560/work/99954508
dc.identifier.other ORCID: /0000-0002-6960-7447/work/99954632
dc.identifier.other ORCID: /0000-0003-0577-8834/work/99957422
dc.identifier.uri http://hdl.handle.net/10138/334347
dc.description.abstract Backgrounds and Aims: APECED is a rare autoimmune disease caused by mutations in the Autoimmune Regulator gene. A significant proportion of patients also have gastrointestinal symptoms, including malabsorption, chronic diarrhea, and obstipation. The pathological background of the gastrointestinal symptoms remains incompletely understood and involves multiple factors, with autoimmunity being the most common underlying cause. Patients with APECED have increased immune responses against gut commensals. Our objective was to evaluate whether the intestinal microbiota composition, predicted functions or fungal abundance differ between Finnish patients with APECED and healthy controls, and whether these associate to the patients’ clinical phenotype and gastrointestinal symptoms. Methods: DNA was isolated from fecal samples from 15 patients with APECED (median age 46.4 years) together with 15 samples from body mass index matched healthy controls. DNA samples were subjected to analysis of the gut microbiota using 16S rRNA gene amplicon sequencing, imputed metagenomics using the PICRUSt2 algorithm, and quantitative PCR for fungi. Extensive correlations of the microbiota with patient characteristics were determined. Results: Analysis of gut microbiota indicated that both alpha- and beta-diversity were altered in patients with APECED compared to healthy controls. The fraction of Faecalibacterium was reduced in patients with APECED while that of Atopobium spp. and several gram-negative genera previously implicated in biofilm formation, e.g. Veillonella, Prevotella, Megasphaera and Heamophilus, were increased in parallel to lipopolysaccharide (LPS) synthesis in imputed metagenomics. The differences in gut microbiota were linked to patient characteristics, especially the presence of anti-Saccharomyces cerevisiae antibodies (ASCA) and severity of gastrointestinal symptoms. Conclusions: Gut microbiota of patients with APECED is altered and enriched with predominantly gram-negative bacterial taxa that may promote biofilm formation and lead to increased exposure to LPS in the patients. The most pronounced alterations in the microbiota were associated with more severe gastrointestinal symptoms. en
dc.format.extent 12
dc.language.iso eng
dc.relation.ispartof Frontiers in Immunology
dc.rights cc_by
dc.rights.uri info:eu-repo/semantics/openAccess
dc.subject ANTIBODIES
dc.subject DISEASE
dc.subject MICROBIOME
dc.subject atopobium
dc.subject autoantibodies
dc.subject autoimmunity
dc.subject dysbiosis
dc.subject faecalibacterium
dc.subject gut microbiota
dc.subject immune dysregulation
dc.subject lps
dc.subject 3121 General medicine, internal medicine and other clinical medicine
dc.title Fecal Bacteria Implicated in Biofilm Production Are Enriched and Associate to Gastrointestinal Symptoms in Patients With APECED - A Pilot Study en
dc.type Article
dc.contributor.organization Department of Bacteriology and Immunology
dc.contributor.organization de Vos & Salonen group
dc.contributor.organization HUMI - Human Microbiome Research
dc.contributor.organization CAMM - Research Program for Clinical and Molecular Metabolism
dc.contributor.organization HUS Children and Adolescents
dc.contributor.organization Clinicum
dc.contributor.organization Lastentautien yksikkö
dc.contributor.organization Children's Hospital
dc.contributor.organization Department of Food and Nutrition
dc.contributor.organization Willem Meindert Vos de / Principal Investigator
dc.contributor.organization Research Programs Unit
dc.contributor.organization Petteri Arstila / Principal Investigator
dc.description.reviewstatus Peer reviewed
dc.relation.doi https://doi.org/10.3389/fimmu.2021.668219
dc.relation.issn 1664-3224
dc.rights.accesslevel openAccess
dc.type.version publishedVersion

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