Sub-100 mu m Spatial Resolution Ambient Mass Spectrometry Imaging of Rodent Brain with Laser Ablation Atmospheric Pressure Photoionization (LAAPPI) and Laser Ablation Electrospray Ionization (LAESI)

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Hieta , J-P , Kopra , J , Räikkönen , H , Kauppila , T & Kostiainen , R 2020 , ' Sub-100 mu m Spatial Resolution Ambient Mass Spectrometry Imaging of Rodent Brain with Laser Ablation Atmospheric Pressure Photoionization (LAAPPI) and Laser Ablation Electrospray Ionization (LAESI) ' , Analytical Chemistry , vol. 92 , no. 20 , pp. 13734-13741 . https://doi.org/10.1021/acs.analchem.0c01597

Title: Sub-100 mu m Spatial Resolution Ambient Mass Spectrometry Imaging of Rodent Brain with Laser Ablation Atmospheric Pressure Photoionization (LAAPPI) and Laser Ablation Electrospray Ionization (LAESI)
Author: Hieta, Juha-Pekka; Kopra, Jaakko; Räikkönen, Heikki; Kauppila, Tiina; Kostiainen, Risto
Contributor organization: Division of Pharmaceutical Chemistry and Technology
Drug Research Program
Divisions of Faculty of Pharmacy
Division of Pharmacology and Pharmacotherapy
Faculty of Pharmacy
Department of Chemistry
VERIFIN
Risto Kostiainen / Principal Investigator
Date: 2020-10-20
Language: eng
Number of pages: 8
Belongs to series: Analytical Chemistry
ISSN: 0003-2700
DOI: https://doi.org/10.1021/acs.analchem.0c01597
URI: http://hdl.handle.net/10138/334367
Abstract: In this study, we applied a new IR laser-beam-focusing technique to enable sub-100 μm spatial resolution in laser ablation atmospheric pressure photoionization (LAAPPI) and laser ablation electrospray ionization (LAESI) mass spectrometry imaging (MSI). After optimization of operational parameters, both LAAPPI- and LAESI-MSI with a spatial resolution of 70 μm produced high-quality MS images, which allowed accurate localization of metabolites and lipids in the mouse and rat brain. Negative and positive ion LAAPPI- and LAESI-MS detected many of the same metabolites and lipids in the brain. Many compounds were also detected either by LAAPPI- or LAESI-MS, indicating that LAAPPI and LAESI are more complementary than alternative methods.In this study, we applied a new IR laser-beamfocusing technique to enable sub-100 mu m spatial resolution in laser ablation atmospheric pressure photoionization (LAAPPI) and laser ablation electrospray ionization (LAESI) mass spectrometry imaging (MSI). After optimization of operational parameters, both LAAPPI- and LAESI-MSI with a spatial resolution of 70 mu m produced high-quality MS images, which allowed accurate localization of metabolites and lipids in the mouse and rat brain. Negative and positive ion LAAPPI- and LAESI-MS detected many of the same metabolites and lipids in the brain. Many compounds were also detected either by LAAPPI- or LAESI-MS, indicating that LAAPPI and LAESI are more complementary than alternative methods.
Subject: 116 Chemical sciences
DESORPTION IONIZATION
TISSUE-SECTIONS
RAT-BRAIN
MATRIX
METABOLOMICS
PEPTIDES
PROTEINS
MYELIN
317 Pharmacy
Peer reviewed: Yes
Usage restriction: openAccess
Self-archived version: acceptedVersion


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