Sub-100 μm Spatial Resolution Ambient Mass Spectrometry Imaging of Rodent Brain with Laser Ablation Atmospheric Pressure Photoionization (LAAPPI) and Laser Ablation Electrospray Ionization (LAESI)

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Hieta , J-P , Kopra , J , Räikkönen , H , Kauppila , T & Kostiainen , R 2020 , ' Sub-100 μm Spatial Resolution Ambient Mass Spectrometry Imaging of Rodent Brain with Laser Ablation Atmospheric Pressure Photoionization (LAAPPI) and Laser Ablation Electrospray Ionization (LAESI) ' , Analytical Chemistry , vol. 92 , no. 20 , pp. 13734-13741 . https://doi.org/10.1021/acs.analchem.0c01597

Title: Sub-100 μm Spatial Resolution Ambient Mass Spectrometry Imaging of Rodent Brain with Laser Ablation Atmospheric Pressure Photoionization (LAAPPI) and Laser Ablation Electrospray Ionization (LAESI)
Author: Hieta, Juha-Pekka; Kopra, Jaakko; Räikkönen, Heikki; Kauppila, Tiina; Kostiainen, Risto
Contributor: University of Helsinki, Division of Pharmaceutical Chemistry and Technology
University of Helsinki, Divisions of Faculty of Pharmacy
University of Helsinki, Faculty of Pharmacy
University of Helsinki, Department of Chemistry
University of Helsinki, Drug Research Program
Date: 2020-09-15
Language: eng
Number of pages: 8
Belongs to series: Analytical Chemistry
ISSN: 0003-2700
URI: http://hdl.handle.net/10138/334367
Abstract: In this study, we applied a new IR laser-beam-focusing technique to enable sub-100 μm spatial resolution in laser ablation atmospheric pressure photoionization (LAAPPI) and laser ablation electrospray ionization (LAESI) mass spectrometry imaging (MSI). After optimization of operational parameters, both LAAPPI- and LAESI-MSI with a spatial resolution of 70 μm produced high-quality MS images, which allowed accurate localization of metabolites and lipids in the mouse and rat brain. Negative and positive ion LAAPPI- and LAESI-MS detected many of the same metabolites and lipids in the brain. Many compounds were also detected either by LAAPPI- or LAESI-MS, indicating that LAAPPI and LAESI are more complementary than alternative methods.
Subject: 116 Chemical sciences
DESORPTION IONIZATION
TISSUE-SECTIONS
RAT-BRAIN
MATRIX
METABOLOMICS
PEPTIDES
PROTEINS
MYELIN
317 Pharmacy
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