Does Day-to-Day Variability in Stool Consistency Link to the Fecal Microbiota Composition?

Show simple item record COST Action GENIEURI BM1106 Microb Vork, Lisa Penders, John Jalanka, Jonna Salonen, Anne de Vos, Willem M. Jonkers, Daisy M. A. E. 2021-09-16T06:02:01Z 2021-09-16T06:02:01Z 2021-07-20
dc.identifier.citation COST Action GENIEURI BM1106 Microb , Vork , L , Penders , J , Jalanka , J , Salonen , A , de Vos , W M & Jonkers , D M A E 2021 , ' Does Day-to-Day Variability in Stool Consistency Link to the Fecal Microbiota Composition? ' , Frontiers in Cellular and Infection Microbiology , vol. 11 , 639667 .
dc.identifier.other PURE: 168507527
dc.identifier.other PURE UUID: 212322df-2412-420a-aec4-3ba2f49cbe5f
dc.identifier.other WOS: 000680709300001
dc.identifier.other ORCID: /0000-0002-3847-8136/work/100080932
dc.identifier.other ORCID: /0000-0002-6960-7447/work/100080993
dc.description.abstract Introduction Stool consistency has been associated with fecal microbial composition. Stool consistency often varies over time, in subjects with and without gastrointestinal disorders, raising the question whether variability in the microbial composition should be considered in microbiota studies. We evaluated within-subject day-to-day variability in stool consistency and the association with the fecal microbiota in irritable bowel syndrome (IBS) and healthy subjects, over seven days. Methods Twelve IBS patients and 12 healthy subjects collected fecal samples during seven consecutive days. Stool consistency was determined by the patient-reported Bristol Stool Scale (BSS) and fecal dry weight percentage. 16S rRNA V4 gene sequencing was performed and microbial richness (alpha diversity; Chao1 index, observed number of species, effective Shannon index) and microbial community structure (beta diversity; Bray-Curtis distance, generalized UniFrac, and taxa abundance on family level) were determined. Results Linear mixed-effects models showed significant associations between stool consistency and microbial richness, but no time effect. This implies that between-subject but not within-subject variation in microbiota over time can partially be explained by variation in stool consistency. Redundancy analysis showed a significant association between stool consistency and microbial community structure, but additional linear mixed-effects models did not demonstrate a time effect on this. Conclusion This study supports an association between stool consistency and fecal microbiota, but no effect of day-to-day fluctuations in stool consistency within seven days. This consolidates the importance of considering stool consistency in gut microbiota research, though confirms the validity of single fecal sampling to represent an individual's microbiota at a given time point. NCT00775060. en
dc.format.extent 10
dc.language.iso eng
dc.relation.ispartof Frontiers in Cellular and Infection Microbiology
dc.rights cc_by
dc.rights.uri info:eu-repo/semantics/openAccess
dc.subject fecal microbiota
dc.subject intestinal microbiome
dc.subject stool consistency
dc.subject irritable bowel syndrome
dc.subject adult
dc.subject 16S RIBOSOMAL-RNA
dc.subject ENTEROTYPES
dc.subject SAMPLES
dc.subject 3111 Biomedicine
dc.subject 11832 Microbiology and virology
dc.title Does Day-to-Day Variability in Stool Consistency Link to the Fecal Microbiota Composition? en
dc.type Article
dc.contributor.organization HUMI - Human Microbiome Research
dc.contributor.organization Faculty of Medicine
dc.contributor.organization University of Helsinki
dc.contributor.organization Faculty Common Matters
dc.contributor.organization Clinicum
dc.contributor.organization de Vos & Salonen group
dc.contributor.organization Department of Bacteriology and Immunology
dc.contributor.organization Willem Meindert Vos de / Principal Investigator
dc.contributor.organization Research Programs Unit
dc.description.reviewstatus Peer reviewed
dc.relation.issn 2235-2988
dc.rights.accesslevel openAccess
dc.type.version publishedVersion

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