Differential polarization and activation dynamics of systemic T helper cell subsets after aneurysmal subarachnoid hemorrhage (SAH) and during post-SAH complications

Show simple item record

dc.contributor.author Chaudhry, Shafqat Rasul
dc.contributor.author Kahlert, Ulf Dietrich
dc.contributor.author Kinfe, Thomas Mehari
dc.contributor.author Endl, Elmar
dc.contributor.author Dolf, Andreas
dc.contributor.author Niemelä, Mika
dc.contributor.author Haenggi, Daniel
dc.contributor.author Muhammad, Sajjad
dc.date.accessioned 2021-09-20T12:57:02Z
dc.date.available 2021-09-20T12:57:02Z
dc.date.issued 2021-07-09
dc.identifier.citation Chaudhry , S R , Kahlert , U D , Kinfe , T M , Endl , E , Dolf , A , Niemelä , M , Haenggi , D & Muhammad , S 2021 , ' Differential polarization and activation dynamics of systemic T helper cell subsets after aneurysmal subarachnoid hemorrhage (SAH) and during post-SAH complications ' , Scientific Reports , vol. 11 , no. 1 , 14226 . https://doi.org/10.1038/s41598-021-92873-x
dc.identifier.other PURE: 168615206
dc.identifier.other PURE UUID: fba94493-d39e-47e4-8394-a2cd40c5b537
dc.identifier.other WOS: 000674491200003
dc.identifier.uri http://hdl.handle.net/10138/334466
dc.description.abstract Aneurysmal subarachnoid hemorrhage (SAH) is associated with high morbidity and mortality. Devastating post-SAH complications, such as cerebral vasospasm (CVS), delayed cerebral ischemia or seizures to mention a few, are mainly responsible for the poor clinical outcome. Inflammation plays an indispensable role during early brain injury (EBI) and delayed brain injury (DBI) phases over which these complications arise. T helper cells are the major cytokine secreting cells of adaptive immunity that can polarize to multiple functionally unique sub-populations. Here, we investigate different CD4+T cell subsets during EBI and DBI phases after SAH, and their dynamics during post-SAH complications. Peripheral venous blood from 15 SAH patients during EBI and DBI phases, was analyzed by multicolour flowcytometry. Different subsets of CD3+CD4+T cells were characterized by differential cell surface expression of CXCR3 and CCR6 into Th1, Th2, Th17, whereas Tregs were defined by CD25(hi)CD127(lo). The analysis of activation states was done by the expression of stable activation markers CD38 and HLA-DR. Interestingly, compared to healthy controls, Tregs were significantly increased during both EBI and DBI phases. Different activation states of Tregs showed differential significant increase during EBI and DBI phases compared to controls. HLA-DR-CD38+Tregs were significantly increased during DBI phase compared to EBI phase in SAH patients developing CVS, seizures and infections. However, HLA-DR-CD38-Tregs were significantly reduced during EBI phase in patients with cerebral ischemia (CI) compared to those without CI. HLA-DR-CD38-Th2 cells were significantly increased during EBI phase compared to controls. A significant reduction in Th17/Tregs and HLA-DR-CD38+Th17/Tregs ratios was observed during both EBI and DBI phases compared to controls. While HLA-DR-CD38-Th17/Tregs and HLA-DR-CD38-Th1/Th2 ratios were impaired only during EBI phase compared to controls. In conclusion, CD4+T cell subsets display dynamic and unique activation patterns after SAH and during the course of the manifestation of post-SAH complications, which may be helpful for the development of precision neurovascular care. However, to claim this, confirmatory studies with larger patient cohorts, ideally from different ethnic backgrounds, are required. Moreover, our descriptive study may be the grounds for subsequent lab endeavors to explore the underlying mechanisms of our observations. en
dc.format.extent 10
dc.language.iso eng
dc.relation.ispartof Scientific Reports
dc.rights cc_by
dc.rights.uri info:eu-repo/semantics/openAccess
dc.subject MECHANISMS
dc.subject CD4(+)
dc.subject 3124 Neurology and psychiatry
dc.subject 3126 Surgery, anesthesiology, intensive care, radiology
dc.title Differential polarization and activation dynamics of systemic T helper cell subsets after aneurysmal subarachnoid hemorrhage (SAH) and during post-SAH complications en
dc.type Article
dc.contributor.organization Department of Neurosciences
dc.contributor.organization HUS Neurocenter
dc.contributor.organization Neurokirurgian yksikkö
dc.contributor.organization University of Helsinki
dc.contributor.organization Clinicum
dc.description.reviewstatus Peer reviewed
dc.relation.doi https://doi.org/10.1038/s41598-021-92873-x
dc.relation.issn 2045-2322
dc.rights.accesslevel openAccess
dc.type.version publishedVersion

Files in this item

Total number of downloads: Loading...

Files Size Format View
s41598_021_92873_x.pdf 1.697Mb PDF View/Open

This item appears in the following Collection(s)

Show simple item record