Depolymerization of biorefinery lignin by improved laccases of the white-rot fungus Obba rivulosa

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dc.contributor.author Wallenius, Janne
dc.contributor.author Kontro, Jussi
dc.contributor.author Lyra, Christina
dc.contributor.author Kuuskeri, Jaana
dc.contributor.author Wan, Xing
dc.contributor.author Kahkonen, Mika A.
dc.contributor.author Baig, Irshad
dc.contributor.author Kamer, Paul C. J.
dc.contributor.author Sipila, Jussi
dc.contributor.author Makela, Miia R.
dc.contributor.author Nousiainen, Paula
dc.contributor.author Hilden, Kristiina
dc.date.accessioned 2021-09-28T14:11:01Z
dc.date.available 2021-09-28T14:11:01Z
dc.date.issued 2021-09
dc.identifier.citation Wallenius , J , Kontro , J , Lyra , C , Kuuskeri , J , Wan , X , Kahkonen , M A , Baig , I , Kamer , P C J , Sipila , J , Makela , M R , Nousiainen , P & Hilden , K 2021 , ' Depolymerization of biorefinery lignin by improved laccases of the white-rot fungus Obba rivulosa ' , Microbial Biotechnology , vol. 14 , no. 5 , pp. 2140-2151 . https://doi.org/10.1111/1751-7915.13896
dc.identifier.other PURE: 167397926
dc.identifier.other PURE UUID: 75588b96-2579-431c-bebc-e9395458d4d4
dc.identifier.other WOS: 000678875300001
dc.identifier.other ORCID: /0000-0001-7551-9956/work/100695611
dc.identifier.other ORCID: /0000-0002-5838-2086/work/100696743
dc.identifier.other ORCID: /0000-0003-1786-4027/work/100697224
dc.identifier.other ORCID: /0000-0003-0771-2329/work/100697592
dc.identifier.uri http://hdl.handle.net/10138/334678
dc.description.abstract Fungal laccases are attracting enzymes for sustainable valorization of biorefinery lignins. To improve the lignin oxidation capacity of two previously characterized laccase isoenzymes from the white-rot fungus Obba rivulosa, we mutated their substrate-binding site at T1. As a result, the pH optimum of the recombinantly produced laccase variant rOrLcc2-D206N shifted by three units towards neutral pH. O. rivulosa laccase variants with redox mediators oxidized both the dimeric lignin model compound and biorefinery poplar lignin. Significant structural changes, such as selective benzylic alpha-oxidation, were detected by nuclear magnetic resonance analysis, although no polymerization of lignin was observed by gel permeation chromatography. This suggests that especially rOrLcc2-D206N is a promising candidate for lignin-related applications. en
dc.format.extent 12
dc.language.iso eng
dc.relation.ispartof Microbial Biotechnology
dc.rights cc_by
dc.rights.uri info:eu-repo/semantics/openAccess
dc.subject 116 Chemical sciences
dc.subject BOND-CLEAVAGE
dc.subject OXIDATION
dc.subject LIGNOCELLULOSE
dc.subject PEROXIDASE
dc.subject MEDIATORS
dc.title Depolymerization of biorefinery lignin by improved laccases of the white-rot fungus Obba rivulosa en
dc.type Article
dc.contributor.organization Department of Microbiology
dc.contributor.organization Department of Chemistry
dc.contributor.organization Fungal Genetics and Biotechnology
dc.contributor.organization Department of Food and Nutrition
dc.contributor.organization Jussi Sipilä / Principal Investigator
dc.contributor.organization Helsinki Institute of Sustainability Science (HELSUS)
dc.description.reviewstatus Peer reviewed
dc.relation.doi https://doi.org/10.1111/1751-7915.13896
dc.relation.issn 1751-7915
dc.rights.accesslevel openAccess
dc.type.version publishedVersion

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