HiPS-Endothelial Cells Acquire Cardiac Endothelial Phenotype in Co-culture With hiPS-Cardiomyocytes

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Helle , E , Ampuja , M , Dainis , A , Antola , L , Temmes , E , Tolvanen , E , Mervaala , E & Kivelä , R 2021 , ' HiPS-Endothelial Cells Acquire Cardiac Endothelial Phenotype in Co-culture With hiPS-Cardiomyocytes ' , Frontiers in Cell and Developmental Biology , vol. 9 , 715093 . https://doi.org/10.3389/fcell.2021.715093

Title: HiPS-Endothelial Cells Acquire Cardiac Endothelial Phenotype in Co-culture With hiPS-Cardiomyocytes
Author: Helle, Emmi; Ampuja, Minna; Dainis, Alexandra; Antola, Laura; Temmes, Elina; Tolvanen, Erik; Mervaala, Eero; Kivelä, Riikka
Other contributor: University of Helsinki, STEMM - Stem Cells and Metabolism Research Program
University of Helsinki, STEMM - Stem Cells and Metabolism Research Program
University of Helsinki, STEMM - Stem Cells and Metabolism Research Program
University of Helsinki, STEMM - Stem Cells and Metabolism Research Program
University of Helsinki, Department of Pharmacology
University of Helsinki, STEMM - Stem Cells and Metabolism Research Program






Date: 2021-08-06
Language: eng
Number of pages: 17
Belongs to series: Frontiers in Cell and Developmental Biology
ISSN: 2296-634X
DOI: https://doi.org/10.3389/fcell.2021.715093
URI: http://hdl.handle.net/10138/334804
Abstract: Cell-cell interactions are crucial for organ development and function. In the heart, endothelial cells engage in bidirectional communication with cardiomyocytes regulating cardiac development and growth. We aimed to elucidate the organotypic development of cardiac endothelial cells and cardiomyocyte and endothelial cell crosstalk using human induced pluripotent stem cells (hiPSC). Single-cell RNA sequencing was performed with hiPSC-derived cardiomyocytes (hiPS-CMs) and endothelial cells (hiPS-ECs) in mono- and co-culture. The presence of hiPS-CMs led to increased expression of transcripts related to vascular development and maturation, cardiac development, as well as cardiac endothelial cell and endocardium-specific genes in hiPS-ECs. Interestingly, co-culture induced the expression of cardiomyocyte myofibrillar genes and MYL7 and MYL4 protein expression was detected in hiPS-ECs. Major regulators of BMP- and Notch-signaling pathways were induced in both cell types in co-culture. These results reflect the findings from animal studies and extend them to human endothelial cells, demonstrating the importance of EC-CM interactions during development.
Subject: human induced pluripotent stem cells
cardiomyocytes
endothelial cells
co-culture
single cell RNA sequencing
GENE-EXPRESSION
HEART
GENERATION
DIFFERENTIATION
MECHANISMS
SIGNATURES
LETHALITY
JUNCTIONS
COMPONENT
EMBRYO
1182 Biochemistry, cell and molecular biology
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