Morales , L O , Shapiguzov , A , Safronov , O , Leppälä , J , Vaahtera , L , Yarmolinsky , D , Kollist , H & Brosche , M 2021 , ' Ozone responses in Arabidopsis : beyond stomatal conductance ' , Plant Physiology , vol. 186 , no. 1 , pp. 180-192 . https://doi.org/10.1093/plphys/kiab097
Title: | Ozone responses in Arabidopsis : beyond stomatal conductance |
Author: | Morales, Luis O.; Shapiguzov, Alexey; Safronov, Omid; Leppälä, Johanna; Vaahtera, Lauri; Yarmolinsky, Dmitry; Kollist, Hannes; Brosche, Mikael |
Contributor organization: | Organismal and Evolutionary Biology Research Programme Viikki Plant Science Centre (ViPS) Plant ROS-Signalling Plant Biology Molecular and Integrative Biosciences Research Programme Plant-Fungal Interactions Group Bioinformatics for Molecular Biology and Genomics (BMBG) Plant stress and natural variation |
Date: | 2021-05 |
Language: | eng |
Number of pages: | 13 |
Belongs to series: | Plant Physiology |
ISSN: | 0032-0889 |
DOI: | https://doi.org/10.1093/plphys/kiab097 |
URI: | http://hdl.handle.net/10138/334916 |
Abstract: | Tropospheric ozone (O-3) is a major air pollutant that decreases yield of important crops worldwide. Despite long-lasting research of its negative effects on plants, there are many gaps in our knowledge on how plants respond to O-3. In this study, we used natural variation in the model plant Arabidopsis (Arabidopsis thaliana) to characterize molecular and physiological mechanisms underlying O-3 sensitivity. A key parameter in models for O-3 damage is stomatal uptake. Here we show that the extent of O-3 damage in the sensitive Arabidopsis accession Shahdara (Sha) does not correspond with O-3 uptake, pointing toward stomata-independent mechanisms for the development of O-3 damage. We compared tolerant (Col-0) versus sensitive accessions (Sha, Cvi-0) in assays related to photosynthesis, cell death, antioxidants, and transcriptional regulation. Acute O-3 exposure increased cell death, development of lesions in the leaves, and decreased photosynthesis in sensitive accessions. In both Sha and Cvi-0, O-3-induced lesions were associated with decreased maximal chlorophyll fluorescence and low quantum yield of electron transfer from Photosystem II to plastoquinone. However, O-3-induced repression of photosynthesis in these two O-3-sensitive accessions developed in different ways. We demonstrate that O-3 sensitivity in Arabidopsis is influenced by genetic diversity given that Sha and Cvi-0 developed accession-specific transcriptional responses to O-3. Our findings advance the understanding of plant responses to O-3 and set a framework for future studies to characterize molecular and physiological mechanisms allowing plants to respond to high O-3 levels in the atmosphere as a result of high air pollution and climate change. |
Subject: |
GENE-EXPRESSION
THALIANA ACCESSIONS OXIDATIVE BURST ABIOTIC STRESS CELL-DEATH PHOTOSYNTHESIS ROLES TRANSCRIPTOME FLUORESCENCE SENSITIVITY 11831 Plant biology |
Peer reviewed: | Yes |
Rights: | cc_by |
Usage restriction: | openAccess |
Self-archived version: | publishedVersion |
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