Ozone responses in Arabidopsis : beyond stomatal conductance

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Morales , L O , Shapiguzov , A , Safronov , O , Leppälä , J , Vaahtera , L , Yarmolinsky , D , Kollist , H & Brosche , M 2021 , ' Ozone responses in Arabidopsis : beyond stomatal conductance ' , Plant Physiology , vol. 186 , no. 1 , pp. 180-192 . https://doi.org/10.1093/plphys/kiab097

Title: Ozone responses in Arabidopsis : beyond stomatal conductance
Author: Morales, Luis O.; Shapiguzov, Alexey; Safronov, Omid; Leppälä, Johanna; Vaahtera, Lauri; Yarmolinsky, Dmitry; Kollist, Hannes; Brosche, Mikael
Contributor organization: Organismal and Evolutionary Biology Research Programme
Viikki Plant Science Centre (ViPS)
Plant ROS-Signalling
Plant Biology
Molecular and Integrative Biosciences Research Programme
Plant-Fungal Interactions Group
Bioinformatics for Molecular Biology and Genomics (BMBG)
Plant stress and natural variation
Date: 2021-05
Language: eng
Number of pages: 13
Belongs to series: Plant Physiology
ISSN: 0032-0889
DOI: https://doi.org/10.1093/plphys/kiab097
URI: http://hdl.handle.net/10138/334916
Abstract: Tropospheric ozone (O-3) is a major air pollutant that decreases yield of important crops worldwide. Despite long-lasting research of its negative effects on plants, there are many gaps in our knowledge on how plants respond to O-3. In this study, we used natural variation in the model plant Arabidopsis (Arabidopsis thaliana) to characterize molecular and physiological mechanisms underlying O-3 sensitivity. A key parameter in models for O-3 damage is stomatal uptake. Here we show that the extent of O-3 damage in the sensitive Arabidopsis accession Shahdara (Sha) does not correspond with O-3 uptake, pointing toward stomata-independent mechanisms for the development of O-3 damage. We compared tolerant (Col-0) versus sensitive accessions (Sha, Cvi-0) in assays related to photosynthesis, cell death, antioxidants, and transcriptional regulation. Acute O-3 exposure increased cell death, development of lesions in the leaves, and decreased photosynthesis in sensitive accessions. In both Sha and Cvi-0, O-3-induced lesions were associated with decreased maximal chlorophyll fluorescence and low quantum yield of electron transfer from Photosystem II to plastoquinone. However, O-3-induced repression of photosynthesis in these two O-3-sensitive accessions developed in different ways. We demonstrate that O-3 sensitivity in Arabidopsis is influenced by genetic diversity given that Sha and Cvi-0 developed accession-specific transcriptional responses to O-3. Our findings advance the understanding of plant responses to O-3 and set a framework for future studies to characterize molecular and physiological mechanisms allowing plants to respond to high O-3 levels in the atmosphere as a result of high air pollution and climate change.
11831 Plant biology
Peer reviewed: Yes
Rights: cc_by
Usage restriction: openAccess
Self-archived version: publishedVersion

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