A novel canine nuclear magnetic resonance spectroscopy-based metabolomics platform : Validation and sample handling

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Ottka , C , Vapalahti , K , Puurunen , J , Vahtera , L & Lohi , H 2021 , ' A novel canine nuclear magnetic resonance spectroscopy-based metabolomics platform : Validation and sample handling ' , Veterinary Clinical Pathology , vol. 50 , no. 3 , pp. 410-426 . https://doi.org/10.1111/vcp.12954

Title: A novel canine nuclear magnetic resonance spectroscopy-based metabolomics platform : Validation and sample handling
Author: Ottka, Claudia; Vapalahti, Katariina; Puurunen, Jenni; Vahtera, Laura; Lohi, Hannes
Other contributor: University of Helsinki, Veterinary Biosciences
University of Helsinki, HUSLAB
University of Helsinki, Hannes Tapani Lohi / Principal Investigator
University of Helsinki, Medicum
University of Helsinki, Helsinki One Health (HOH)







Date: 2021-09
Language: eng
Number of pages: 17
Belongs to series: Veterinary Clinical Pathology
ISSN: 0275-6382
DOI: https://doi.org/10.1111/vcp.12954
URI: http://hdl.handle.net/10138/335051
Abstract: Background Metabolomics has been proven to be an invaluable research tool by providing comprehensive insight into systemic metabolism. However, the lack of scalable and quantitative methods with known reference intervals (RIs) and documented reproducibility has prevented the use of metabolomics in the clinical setting. Objective The objective of this study was to validate the developed quantitative nuclear magnetic resonance (NMR) spectroscopy-based metabolomics platform for canine serum and plasma samples and determine optimal sample handling conditions for its use. Methods Altogether, 8247 canine samples were analyzed using a Bruker's 500 MHz NMR spectrometer. Using statistical approaches derived from international guidelines, we studied method precision, measurand stability in various long- and short-term storage conditions, as well as the effect of prolonged contact with red blood cells (RBCs), and differences among blood collection tubes. We also screened interferences with lipemia, hemolysis, and bilirubinemia. The results were compared against routine clinical chemistry methods, and RIs were defined for all measurands. Results We determined RIs for 123 measurands, most of which were previously unpublished. The reproducibility of the results of the NMR platform appeared generally outstanding, and the integrity of the results can be ensured by following standard blood drawing and processing guidelines. Conclusions Owing to the advantages of quantitative results, high reproducibility, and scalability, this canine metabolomics platform holds great potential for numerous clinical and research applications to improve canine health and well-being.
Subject: dog
precision
reference intervals
stability
storage
CLINICAL-CHEMISTRY
GUIDELINES
PROFILES
SYSTEMS
RISK
413 Veterinary science
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