Activated matrix metalloproteinase 8 in serum predicts severity of acute pancreatitis

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Turunen , A , Kuuliala , K , Kuuliala , A , Tervahartiala , T , Mustonen , H , Puolakkainen , P , Kylänpää , A L & Sorsa , T 2021 , ' Activated matrix metalloproteinase 8 in serum predicts severity of acute pancreatitis ' , Pancreatology , vol. 21 , no. 5 , pp. 862-869 .

Title: Activated matrix metalloproteinase 8 in serum predicts severity of acute pancreatitis
Author: Turunen, A.; Kuuliala, K.; Kuuliala, A.; Tervahartiala, T.; Mustonen, H.; Puolakkainen, P.; Kylänpää, A. L.; Sorsa, T.
Contributor organization: University of Helsinki
Department of Bacteriology and Immunology
Helsinki University Hospital Area
HUS Head and Neck Center
Department of Oral and Maxillofacial Diseases
HUS Abdominal Center
Department of Surgery
II kirurgian klinikka
Teachers' Academy
Pauli Puolakkainen / Principal Investigator
Date: 2021-08
Language: eng
Number of pages: 8
Belongs to series: Pancreatology
ISSN: 1424-3903
Abstract: Objectives: Severe acute pancreatitis (SAP) has high morbidity and mortality but there are no widely accepted predictive biomarkers in clinical use. Matrix metalloproteinases (MMPs) are active in tissue destruction and inflammatory responses. We studied whether serum levels of activated MMP-8 (aMMP8), MMP-9 and their regulators tissue inhibitor of matrix metalloproteinases (TIMP)-1, myeloperoxidase (MPO) and human neutrophil elastase (HNE) could predict the development of SAP. Methods: The study comprised 214 AP patients (revised Atlanta classification: 142 mild, MAP; 54 moderately severe, MSAP; 18 SAP) referred to Helsinki University Hospital. A venous blood sample was taken within 72 h from the onset of symptoms. Serum levels of aMMP-8 were determined using immunofluorometric assay, and those of MMP-9, TIMP-1, MPO and HNE using enzyme-linked immunosorbent assay. AP groups were compared using Jonckheere-Terpstra test and predictive value for SAP was analyzed using receiver operating characteristics (ROC) analysis. Results: Serum aMMP-8 levels were higher in SAP (median 657 ng/ml, interquartile range 542-738 ng/ ml) compared to MSAP (358 ng/ml, 175-564 ng/ml; p < 0.001) and MAP (231 ng/ml, 128-507 ng/ml; p < 0.001). Similar trend was seen with TIMP-1 and MPO. In ROC analysis aMMP-8, MPO and TIMP-1 emerged as potential markers for the development of SAP (areas under ROC curves 0.83, 0.71 and 0.69, respectively). Conclusions: Serum aMMP-8 measured early in the course of AP (within 72 h of symptom onset) predicted the development of SAP. (c) 2021 The Authors. Published by Elsevier B.V. on behalf of IAP and EPC. This is an open access article under the CC BY-NC-ND license (
Subject: Acute pancreatitis
Organ dysfunction
Matrix metalloproteinase 8
3121 General medicine, internal medicine and other clinical medicine
Peer reviewed: Yes
Rights: cc_by_nc_nd
Usage restriction: openAccess
Self-archived version: publishedVersion

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