Periodontitis and peri-implantitis tissue levels of Treponema denticola-CTLP and its MMP-8 activating ability

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Petain , S , Kasnak , G , Firatli , E , Tervahartiala , T , Gürsoy , U K & Sorsa , T 2021 , ' Periodontitis and peri-implantitis tissue levels of Treponema denticola-CTLP and its MMP-8 activating ability ' , Acta histochemica : a journal of structural biochemistry, cell and tissue imaging , vol. 123 , no. 6 , 151767 . https://doi.org/10.1016/j.acthis.2021.151767

Title: Periodontitis and peri-implantitis tissue levels of Treponema denticola-CTLP and its MMP-8 activating ability
Author: Petain, Sami; Kasnak, Gokhan; Firatli, Erhan; Tervahartiala, Taina; Gürsoy, Ulvi K.; Sorsa, Timo
Contributor organization: HUS Head and Neck Center
Clinicum
Department of Oral and Maxillofacial Diseases
Date: 2021-09
Language: eng
Number of pages: 6
Belongs to series: Acta histochemica : a journal of structural biochemistry, cell and tissue imaging
ISSN: 0065-1281
DOI: https://doi.org/10.1016/j.acthis.2021.151767
URI: http://hdl.handle.net/10138/335351
Abstract: Background and Aims: Chymotrypsin-like-proteinase of Treponema denticola (Td-CTLP) can stimulate the protein expression and activation of matrix metalloproteinase (MMP)-8 (or collagenase-2), a potent tissue destructive enzyme from gingival cells in vitro. The aims of this study were 1) to demonstrate the proMMP-8 (or latent MMP-8) activation by Td-CTLP in vitro and 2) to detect Td-CTLP and MMP-8 protein levels in the tissue samples of peri-implantitis and periodontitis patients. Materials and Methods: proMMP-8 activation by Td-CTLP was analyzed by immunoblots. Tissue specimens were collected from 38 systemically healthy and non-smoking patients; 14 of whom had moderate to severe periodontitis, 10 of whom were suffering from peri-implantitis, and finally 14 of whom showed no sign of periodontal inflammation nor radiological bone decay (control group). The immune-expression levels of MMP-8 and Td-CTLP in the epithelium and the connective tissue were analyzed immunohistochemically. A pixel color-intensity analyze was performed with ImageJ software (version 1.46c; Rasband WS, National Institutes of Health, Bethesda, MD, USA) to obtain a comparable numeral score for each patient's epithelium and connective tissue MMP-8 and Td-CTLP enzyme level. Results: Td-CTLP activated proMMP-8 in vitro by converting the 70 75 kDa proMMP-8 to 65 kDa active MMP-8. Also, lower molecular size 25 50 kDa parts of MMP-8 were formed. There was no statistically significant difference between the study groups in terms of their MMP-8 and Td-CTLP levels in the epithelium or in the connective tissue. Conclusion: Regarding the limits of this study, it can thus be said that the Td-CTLP enzyme can activate the host proMMP-8 enzyme. Tissue protein levels of MMP-8 and Td-CTLP do not seem to be changed in peri-implantitis and in periodontitis.
Subject: MMP-8
collagenase-2
Td-CTLP
Treponema denticola
Periodontitis
Peri-implantitis
CONSENSUS REPORT
BIOMARKERS
PROTEINASE
EXPRESSION
DISEASES
CELLS
313 Dentistry
1182 Biochemistry, cell and molecular biology
Peer reviewed: Yes
Rights: cc_by
Usage restriction: openAccess
Self-archived version: publishedVersion


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