Effect of Methyl Jasmonate on Thymol, Carvacrol, Phytochemical Accumulation, and Expression of Key Genes Involved in Thymol/Carvacrol Biosynthetic Pathway in Some Iranian Thyme Species

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Kianersi, F.; Pour-Aboughadareh, A.; Majdi, M.; Poczai, P. Effect of Methyl Jasmonate on Thymol, Carvacrol, Phytochemical Accumulation, and Expression of Key Genes Involved in Thymol/Carvacrol Biosynthetic Pathway in Some Iranian Thyme Species. Int. J. Mol. Sci. 2021, 22, 11124.

Title: Effect of Methyl Jasmonate on Thymol, Carvacrol, Phytochemical Accumulation, and Expression of Key Genes Involved in Thymol/Carvacrol Biosynthetic Pathway in Some Iranian Thyme Species
Author: Kianersi, Farzad; Pour-Aboughadareh, Alireza; Majdi, Mohammad; Poczai, Peter
Publisher: Multidisciplinary Digital Publishing Institute
Date: 2021-10-15
URI: http://hdl.handle.net/10138/335599
Abstract: Thyme species are a good source of thymol and carvacrol, which play a key role in controlling diseases. For the first time, the expression patterns of γ-terpinene synthase (TPS2), <i>CYP71D178</i>, and <i>CYP71D180</i> genes and the amount of phenolics compounds were evaluated in <i>T. migricus</i> and <i>T. daenensis</i> after different methyl jasmonate (MeJA) treatments. The highest thymol and carvacrol contents were observed in <i>T. migricus</i> (86.27%) and <i>T. daenensis</i> (17.87%) at MeJA 100 µM, which was consistent with the expression patterns of the three investigated genes. All species treated showed high total phenolic and flavonoid content compared to control plants for which the highest amounts were observed in <i>T. vulgaris</i> treated with 100 µM and 10 µM MeJA. Furthermore, in the 100 µM MeJA treatment, the relative expression of <i>TPS2</i> and <i>CYP71D178</i> in <i>T. migricus</i> increased 7.47 and 9.86-fold compared with the control, respectively. The highest level of <i>CYP71D180</i> transcripts (5.15-fold) was also observed for <i>T. daenensis</i> treated. This finding highlights the notion that thymol was known as the dominant component of the essential oil rather than carvacrol in diffident thyme species. This implies that MeJA at different concentrations influenced metabolic pathways and induced expression changes, resulting in a rise in essential oil levels.


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