Thermocontrolled Reversible Enzyme Complexation-Inactivation-Protection by Poly(N-acryloyl glycinamide)

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dc.contributor.author Semenyuk, Pavel I.
dc.contributor.author Kurochkina, Lidia P.
dc.contributor.author Mäkinen, Lauri
dc.contributor.author Muronetz, Vladimir I.
dc.contributor.author Hietala, Sami
dc.date.accessioned 2021-10-22T13:55:57Z
dc.date.available 2021-10-22T13:55:57Z
dc.date.issued 2021-10-19
dc.identifier.citation Semenyuk, P.I.; Kurochkina, L.P.; Mäkinen, L.; Muronetz, V.I.; Hietala, S. Thermocontrolled Reversible Enzyme Complexation-Inactivation-Protection by Poly(N-acryloyl glycinamide). Polymers 2021, 13, 3601.
dc.identifier.uri http://hdl.handle.net/10138/335607
dc.description.abstract A prospective technology for reversible enzyme complexation accompanied with its inactivation and protection followed by reactivation after a fast thermocontrolled release has been demonstrated. A thermoresponsive polymer with upper critical solution temperature, poly(<i>N</i>-acryloyl glycinamide) (PNAGA), which is soluble in water at elevated temperatures but phase separates at low temperatures, has been shown to bind lysozyme, chosen as a model enzyme, at a low temperature (10 °C and lower) but not at room temperature (around 25 °C). The cooling of the mixture of PNAGA and lysozyme solutions from room temperature resulted in the capturing of the protein and the formation of stable complexes; heating it back up was accompanied by dissolving the complexes and the release of the bound lysozyme. Captured by the polymer, lysozyme was inactive, but a temperature-mediated release from the complexes was accompanied by its reactivation. Complexation also partially protected lysozyme from proteolytic degradation by proteinase K, which is useful for biotechnological applications. The obtained results are relevant for important medicinal tasks associated with drug delivery such as the delivery and controlled release of enzyme-based drugs.
dc.publisher Multidisciplinary Digital Publishing Institute
dc.title Thermocontrolled Reversible Enzyme Complexation-Inactivation-Protection by Poly(N-acryloyl glycinamide)
dc.date.updated 2021-10-22T13:55:57Z
dc.type.uri http://purl.org/eprint/entityType/JournalArticle
dc.type.uri http://purl.org/eprint/entityType/Expression
dc.type.uri http://purl.org/eprint/entityType/Expression

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