Evaluation of the effect of donor weight on adipose stromal/stem cell characteristics by using weight-discordant monozygotic twin pairs

Show full item record



Permalink

http://hdl.handle.net/10138/335750

Citation

Juntunen , M , Heinonen , S , Huhtala , H , Rissanen , A , Kaprio , J , Kuismanen , K , Pietiläinen , K H , Miettinen , S & Patrikoski , M 2021 , ' Evaluation of the effect of donor weight on adipose stromal/stem cell characteristics by using weight-discordant monozygotic twin pairs ' , Stem Cell Research & Therapy , vol. 12 , no. 1 , 516 . https://doi.org/10.1186/s13287-021-02587-0

Title: Evaluation of the effect of donor weight on adipose stromal/stem cell characteristics by using weight-discordant monozygotic twin pairs
Author: Juntunen, Miia; Heinonen, Sini; Huhtala, Heini; Rissanen, Aila; Kaprio, Jaakko; Kuismanen, Kirsi; Pietiläinen, Kirsi H.; Miettinen, Susanna; Patrikoski, Mimmi
Other contributor: University of Helsinki, CAMM - Research Program for Clinical and Molecular Metabolism
University of Helsinki, HUS Psychiatry
University of Helsinki, Institute for Molecular Medicine Finland
University of Helsinki, HUS Abdominal Center
University of Helsinki, CAMM - Research Program for Clinical and Molecular Metabolism








Date: 2021-09-26
Language: eng
Number of pages: 13
Belongs to series: Stem Cell Research & Therapy
ISSN: 1757-6512
DOI: https://doi.org/10.1186/s13287-021-02587-0
URI: http://hdl.handle.net/10138/335750
Abstract: Background Adipose stromal/stem cells (ASCs) are promising candidates for future clinical applications. ASCs have regenerative capacity, low immunogenicity, and immunomodulatory ability. The success of future cell-based therapies depends on the appropriate selection of donors. Several factors, including age, sex, and body mass index (BMI), may influence ASC characteristics. Our aim was to investigate the effect of acquired weight on ASC characteristics under the same genetic background using ASCs derived from monozygotic (MZ) twin pairs. Methods ASCs were isolated from subcutaneous adipose tissue from five weight-discordant (WD, within-pair difference in BMI > 3 kg/m(2)) MZ twin pairs, with measured BMI and metabolic status. The ASC immunophenotype, proliferation and osteogenic and adipogenic differentiation capacity were studied. ASC immunogenicity, immunosuppression capacity and the expression of inflammation markers were investigated. ASC angiogenic potential was assessed in cocultures with endothelial cells. Results ASCs showed low immunogenicity, proliferation, and osteogenic differentiation capacity independent of weight among all donors. ASCs showed a mesenchymal stem cell-like immunophenotype; however, the expression of CD146 was significantly higher in leaner WD twins than in heavier cotwins. ASCs from heavier twins from WD pairs showed significantly greater adipogenic differentiation capacity and higher expression of TNF and lower angiogenic potential compared with their leaner cotwins. ASCs showed immunosuppressive capacity in direct cocultures; however, heavier WD twins showed stronger immunosuppressive capacity than leaner cotwins. Conclusions Our genetically matched data suggest that a higher weight of the donor may have some effect on ASC characteristics, especially on angiogenic and adipogenic potential, which should be considered when ASCs are used clinically.
Subject: Adipose stem cells
Cell surface markers
Differentiation
Immunogenicity
Immunosuppression
Proliferation
STEM-CELLS
OSTEOGENIC DIFFERENTIATION
OBESE-PATIENTS
CULTURE
PROLIFERATION
CAPACITY
ALTERS
REGION
YOUNG
1182 Biochemistry, cell and molecular biology
3121 General medicine, internal medicine and other clinical medicine
Rights:


Files in this item

Total number of downloads: Loading...

Files Size Format View
Evaluation_of_the_effect_of_donor_weigh...pdf 4.027Mb PDF View/Open

This item appears in the following Collection(s)

Show full item record