Multiplexed analysis of amino acids in mice brain microdialysis samples using isobaric labeling and liquid chromatography-high resolution tandem mass spectrometry

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dc.contributor.author Heininen, Juho
dc.contributor.author Julku, Ulrika
dc.contributor.author Myöhänen, Timo
dc.contributor.author Kotiaho, Tapio
dc.contributor.author Kostiainen, Risto
dc.date.accessioned 2021-10-28T08:18:03Z
dc.date.available 2021-10-28T08:18:03Z
dc.date.issued 2021-10-25
dc.identifier.citation Heininen , J , Julku , U , Myöhänen , T , Kotiaho , T & Kostiainen , R 2021 , ' Multiplexed analysis of amino acids in mice brain microdialysis samples using isobaric labeling and liquid chromatography-high resolution tandem mass spectrometry ' , Journal of Chromatography. A , vol. 1656 , 462537 . https://doi.org/10.1016/j.chroma.2021.462537
dc.identifier.other PURE: 169851314
dc.identifier.other PURE UUID: dcf4860d-1ba1-4acf-a13b-abab3e9bf7e9
dc.identifier.other WOS: 000708089700001
dc.identifier.other ORCID: /0000-0003-0382-8578/work/102234577
dc.identifier.other ORCID: /0000-0003-2454-4574/work/102235232
dc.identifier.other ORCID: /0000-0002-9277-6687/work/102235404
dc.identifier.uri http://hdl.handle.net/10138/335776
dc.description.abstract We developed a new multiplexed reversed phase liquid chromatography-high resolution tandem mass spectrometric (LC-MS/MS) method. The method is based on isobaric labeling with a tandem mass tag (TMT10-plex) and stable isotope-labeled internal standards, and was used to analyze amino acids in mouse brain microdialysis samples. The TMT10-plex labeling of amino acids allowed analysis of ten samples in one LC-MS/MS run, significantly increasing the sample throughput. The method provides good chromatographic performance (peak half-width between 0.04-0.12 min), allowing separation of all TMTlabeled amino acids with acceptable resolution and high sensitivity (limits of detection typically around 10 nM). The use of stable isotope-labeled internal standards, together with TMT10-plex labeling, ensured good repeatability (relative standard deviation 0.994), indicating good quantitative performance of the multiplexed method. The method was applied to study the effect of d-amphetamine microdialysis perfusion on amino acid concentrations in the mouse brain. All amino acids were reliably detected and quantified, indicating that the method is sensitive enough to detect low concentrations of amino acids in brain microdialysis samples. (c) 2021 The Author(s). Published by Elsevier B.V. This is an open access article under the CC BY license ( http://creativecommons.org/licenses/by/4.0/ ) en
dc.format.extent 10
dc.language.iso eng
dc.relation.ispartof Journal of Chromatography. A
dc.rights cc_by
dc.rights.uri info:eu-repo/semantics/openAccess
dc.subject Multiplexing
dc.subject Isobaric labeling
dc.subject Isotope dilution
dc.subject Metabolites
dc.subject Amino acids
dc.subject High resolution tandem mass spectrometry
dc.subject D-AMPHETAMINE
dc.subject QUANTITATIVE PROTEOMICS
dc.subject EXTRACELLULAR LEVELS
dc.subject LC-MS
dc.subject RAT
dc.subject GLUTAMATE
dc.subject METABOLITES
dc.subject INCREASES
dc.subject STRIATUM
dc.subject GABA
dc.subject 1182 Biochemistry, cell and molecular biology
dc.subject 116 Chemical sciences
dc.title Multiplexed analysis of amino acids in mice brain microdialysis samples using isobaric labeling and liquid chromatography-high resolution tandem mass spectrometry en
dc.type Article
dc.contributor.organization Division of Pharmaceutical Chemistry and Technology
dc.contributor.organization Drug Research Program
dc.contributor.organization Division of Pharmacology and Pharmacotherapy
dc.contributor.organization Regenerative pharmacology group
dc.contributor.organization PREP in neurodegenerative disorders
dc.contributor.organization Faculty of Pharmacy
dc.contributor.organization Divisions of Faculty of Pharmacy
dc.contributor.organization Department of Chemistry
dc.contributor.organization Tapio Kotiaho / Principal Investigator
dc.contributor.organization University Management
dc.contributor.organization Risto Kostiainen / Principal Investigator
dc.description.reviewstatus Peer reviewed
dc.relation.doi https://doi.org/10.1016/j.chroma.2021.462537
dc.relation.issn 0021-9673
dc.rights.accesslevel openAccess
dc.type.version publishedVersion

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