Challenges for the Applications of Human Pluripotent Stem Cell-Derived Liver Organoids

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dc.contributor.author Chang, Mingyang
dc.contributor.author Bogacheva, Mariia S.
dc.contributor.author Lou, Yan-Ru
dc.date.accessioned 2021-10-28T08:25:01Z
dc.date.available 2021-10-28T08:25:01Z
dc.date.issued 2021-10-01
dc.identifier.citation Chang , M , Bogacheva , M S & Lou , Y-R 2021 , ' Challenges for the Applications of Human Pluripotent Stem Cell-Derived Liver Organoids ' , Frontiers in Cell and Developmental Biology , vol. 9 , 748576 . https://doi.org/10.3389/fcell.2021.748576
dc.identifier.other PURE: 169851538
dc.identifier.other PURE UUID: b477003f-179a-4138-94e9-5873538aafcf
dc.identifier.other WOS: 000707796300001
dc.identifier.other ORCID: /0000-0001-7717-6010/work/102233866
dc.identifier.other ORCID: /0000-0001-6608-1120/work/102237359
dc.identifier.uri http://hdl.handle.net/10138/335777
dc.description.abstract The current organoid culture systems allow pluripotent and adult stem cells to self-organize to form three-dimensional (3D) structures that provide a faithful recapitulation of the architecture and function of in vivo organs. In particular, human pluripotent stem cell-derived liver organoids (PSC-LOs) can be used in regenerative medicine and preclinical applications, such as disease modeling and drug discovery. New bioengineering tools, such as microfluidics, biomaterial scaffolds, and 3D bioprinting, are combined with organoid technologies to increase the efficiency of hepatic differentiation and enhance the functional maturity of human PSC-LOs by precise control of cellular microenvironment. Long-term stabilization of hepatocellular functions of in vitro liver organoids requires the combination of hepatic endodermal, endothelial, and mesenchymal cells. To improve the biological function and scalability of human PSC-LOs, bioengineering methods have been used to identify diverse and zonal hepatocyte populations in liver organoids for capturing heterogeneous pathologies. Therefore, constructing engineered liver organoids generated from human PSCs will be an extremely versatile tool in in vitro disease models and regenerative medicine in future. In this review, we aim to discuss the recent advances in bioengineering technologies in liver organoid culture systems that provide a timely and necessary study to model disease pathology and support drug discovery in vitro and to generate cell therapy products for transplantation. en
dc.format.extent 17
dc.language.iso eng
dc.relation.ispartof Frontiers in Cell and Developmental Biology
dc.rights cc_by
dc.rights.uri info:eu-repo/semantics/openAccess
dc.subject human pluripotent stem cells
dc.subject liver organoid
dc.subject regenerative medicine
dc.subject disease modeling
dc.subject drug development
dc.subject LONG-TERM EXPANSION
dc.subject EXTRACELLULAR-MATRIX SCAFFOLDS
dc.subject HEPATOCYTE-LIKE CELLS
dc.subject IN-VIVO MODEL
dc.subject HEPATIC ORGANOIDS
dc.subject COPY NUMBER
dc.subject DIFFERENTIATION
dc.subject GENERATION
dc.subject ZONATION
dc.subject CULTURE
dc.subject 317 Pharmacy
dc.subject 1182 Biochemistry, cell and molecular biology
dc.title Challenges for the Applications of Human Pluripotent Stem Cell-Derived Liver Organoids en
dc.type Review Article
dc.contributor.organization Department of Virology
dc.contributor.organization Tissue engineering for drug research
dc.contributor.organization Division of Pharmaceutical Biosciences
dc.description.reviewstatus Peer reviewed
dc.relation.doi https://doi.org/10.3389/fcell.2021.748576
dc.relation.issn 2296-634X
dc.rights.accesslevel openAccess
dc.type.version publishedVersion

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